Mutant proteins and methods for producing them
    2.
    发明授权
    Mutant proteins and methods for producing them 有权
    突变蛋白及其生产方法

    公开(公告)号:US08703915B2

    公开(公告)日:2014-04-22

    申请号:US13379872

    申请日:2010-06-22

    IPC分类号: C07K14/705 C12P21/00

    CPC分类号: C07K14/705

    摘要: A method for producing a mutant G-protein coupled receptor (GPCR) with increased stability relative to a parent GPCR, the method comprising making one or more mutations in the amino acid sequence that defines a parent GPCR, wherein (i) the one or more mutations are located within a window of/plus or minus 5 residues, where/is the position of amino acid residue 2.46 in the parent GPCR when the parent GPCR is a Class 1 GPCR, or where/is the position of an equivalent amino acid residue in the parent GPCR when the parent GPCR is a Class 2 or 3 GPCR, and/or (ii) the one or more mutations are located within an amino acid sequence of transmembrane helix 7 in the parent GPCR which amino acid sequence interacts with the window of/plus or minus 5 residues, to provide one or more mutants of the parent GPCR with increased stability.

    摘要翻译: 一种相对于亲本GPCR产生具有增加的稳定性的突变型G蛋白偶联受体(GPCR)的方法,所述方法包括在限定亲本GPCR的氨基酸序列中制备一个或多个突变,其中(i)所述一个或多个 突变位于/正或负5个残基的窗口内,其中当母体GPCR为1类GPCR时,母体GPCR中氨基酸残基2.46的位置,或其中/是等位氨基酸残基的位置 当母体GPCR为2或3 GPCR时,在母体GPCR中,和/或(ii)一个或多个突变位于母体GPCR中的跨膜螺旋7的氨基酸序列内,该氨基酸序列与窗口相互作用 的/加或减5个残基,以提供母体GPCR的一种或多种突变体具有增加的稳定性。

    MUTANT PROTEINS AND METHODS FOR PRODUCING THEM
    3.
    发明申请
    MUTANT PROTEINS AND METHODS FOR PRODUCING THEM 有权
    突变蛋白及其生产方法

    公开(公告)号:US20120165507A1

    公开(公告)日:2012-06-28

    申请号:US13379872

    申请日:2010-06-22

    IPC分类号: C07K14/705 C12P21/00

    CPC分类号: C07K14/705

    摘要: A method for producing a mutant G-protein coupled receptor (GPCR) with increased stability relative to a parent GPCR, the method comprising making one or more mutations in the amino acid sequence that defines a parent GPCR, wherein (i) the one or more mutations are located within a window of / plus or minus 5 residues, where / is the position of amino acid residue 2.46 in the parent GPCR when the parent GPCR is a Class 1 GPCR, or where / is the position of an equivalent amino acid residue in the parent GPCR when the parent GPCR is a Class 2 or 3 GPCR, and/or (ii) the one or more mutations are located within an amino acid sequence of transmembrane helix 7 in the parent GPCR which amino acid sequence interacts with the window of / plus or minus 5 residues, to provide one or more mutants of the parent GPCR with increased stability.

    摘要翻译: 一种相对于亲本GPCR产生具有增加的稳定性的突变型G蛋白偶联受体(GPCR)的方法,所述方法包括在限定亲本GPCR的氨基酸序列中制备一个或多个突变,其中(i)所述一个或多个 突变位于/正或负5个残基的窗口内,其中当母体GPCR为1类GPCR时,母体GPCR中氨基酸残基2.46的位置,或其中/是等位氨基酸残基的位置 当母体GPCR为2或3 GPCR时,在母体GPCR中,和/或(ii)一个或多个突变位于母体GPCR中的跨膜螺旋7的氨基酸序列内,该氨基酸序列与窗口相互作用 的/加或减5个残基,以提供母体GPCR的一种或多种突变体具有增加的稳定性。