公开(公告)号：US07052865B1

公开(公告)日：2006-05-30

申请号：US09914191

申请日：2000-02-28

申请人： Hugh Redmond Brady ,  Catherine Mary Godson ,  Finian Mary Martin ,  Ruth Anne McMahon ,  Madeline Anne Murphy

发明人： Hugh Redmond Brady ,  Catherine Mary Godson ,  Finian Mary Martin ,  Ruth Anne McMahon ,  Madeline Anne Murphy

IPC分类号： C12Q1/00 ,  C12Q1/02 ,  C07H21/04

CPC分类号： C12Q1/6809

摘要： A method for identifying a gene having a role in the presentation of diabetic nephropathy comprises culturing mesangial cells in the presence of a concentration of glucose sufficient to induce differential expression, especially up-regulation, of a gene susceptible to such differential expression and identifying the gene so induced. The cells are also optionally subjected to mechanical strain and/or TGF-β1 can be added to the culture medium. The differentially expressed genes can be identified by suppression subtractive hybridisation. The method has resulted in the Identification of novel genes which play a role in the presentation of diabetic nephropathy. The genes can be used as diagnostic markers for diabetic nephropathy and as the basis of drug development programmes.

摘要翻译： 用于鉴定在糖尿病肾病呈现中具有作用的基因的方法包括在足以诱导对这种差异表达敏感的基因的差异表达，特别是上调的葡萄糖浓度存在下培养肾小球系膜细胞，并鉴定该基因 所以诱导。 细胞还任选地受到机械应变，和/或可以将TGF-β1加入到培养基中。 差异表达的基因可以通过抑制消减杂交鉴定。 该方法导致了鉴定在糖尿病肾病呈现中发挥作用的新基因。 该基因可用作糖尿病肾病的诊断标志物，并作为药物开发方案的基础。