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公开(公告)号:US11473115B2
公开(公告)日:2022-10-18
申请号:US17265097
申请日:2020-11-11
申请人: Hunan Plant Protection Institute , Changsha Agreen Bio-Tech Ltd., Co. , Suzhou Ace Chemical Technology Co., Ltd.
发明人: Yong Liu , Pin Su , Zhongying Zhai , Zhuo Zhang , Haixing Yang , Jianping Dai , Bo Zhou , Deyong Zhang , Ju'e Cheng
摘要: The invention discloses an exopolysaccharide from Rhodopseudomonas palustris and a method for preparing and use thereof, and the method for preparing comprises the steps of: 1) keeping a seed solution from Rhodopseudomonas palustris GJ-22 in a fermentation medium for fermentation culture to obtain a fermentation broth; 2) centrifuging the fermentation broth to take the supernatant, which is treated by alcohol precipitation after filtration, and then collecting the pellet from alcohol precipitation by centrifugation to obtain crude polysaccharide; 3) removing proteins from the rude polysaccharide using protease enzymolysis method and Sevag method, followed by dialysis treatment with distilled water to remove small molecules and organic solvent to obtain a polysaccharide sample; 4) purifying the polysaccharide sample through an anion exchange column and a molecular exclusion chromatography column obtain the exopolysaccharide from Rhodopseudomonas palustris.