公开(公告)号：US20200216890A1

公开(公告)日：2020-07-09

申请号：US16723315

申请日：2019-12-20

Applicant: IMEC VZW

Inventor： Koen Martens ,  Pol Van Dorpe ,  Karolien Jans ,  Gabrielle Woronoff

IPC: C12Q1/6869

Abstract: A method for sequencing a template polynucleotide, comprising the steps of: a) Providing a sensor comprising: an active region comprising a source region, a drain region, and a channel region, a dielectric region on the channel region, a polymerase coupled to the dielectric region, the polymerase having an active site, the polymerase being separated from the dielectric region by a gap, one or more sensitizing means, a fluidic gate region to which the polymerase is exposed, a template polynucleotide bound to a primer, the template polynucleotide being bound to the polymerase; b) Exposing the polymerase to one or more nucleotide polyphosphates; and c) Electrically monitoring changes in the channel region electrical properties.