公开(公告)号：US20160341718A1

公开(公告)日：2016-11-24

申请号：US15112475

申请日：2015-01-20

申请人： INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) ,  UNIVERSITÉ PARIS - SUD

发明人： Roberto MALLONE ,  Victor APPAY ,  Anna LISSINA

IPC分类号： G01N33/50 ,  A61K39/00 ,  C12N5/0783 ,  C12N5/095 ,  C12N5/0784

CPC分类号： G01N33/505 ,  A61K39/0011 ,  C12N5/0636 ,  C12N5/0639 ,  C12N5/0695 ,  C12N2500/84 ,  C12N2501/06 ,  C12N2501/22 ,  C12N2501/2301 ,  C12N2501/2304 ,  C12N2501/2307 ,  C12N2501/24 ,  C12N2501/25 ,  C12N2501/999

摘要： The present invention relates to methods for testing T cell priming efficacy in a subject. In particular the present invention relates to an in vitro method for testing T cell priming efficacy in a subject comprising the steps of a) providing sample from the subject, b) culturing the sample in a medium which induces the differentiation of dendritic cells, c) maturing the dendritic cells obtained at step a) in presence of an amount of at least one antigen and an amount of at least one cytokine or ligand suitable for the activation of a pathogen recognition receptor, d) priming and expanding the T cells present in the sample and e) analyzing the func tionality of the primed T cells.

摘要翻译： 本发明涉及在受试者中测试T细胞引发功效的方法。 特别地，本发明涉及一种用于在受试者中测试T细胞引发功效的体外方法，包括以下步骤：a）从受试者提供样品，b）在诱导树突状细胞分化的培养基中培养样品，c） 使步骤a）获得的树突状细胞在至少一种抗原和至少一种适合于激活病原体识别受体的细胞因子或配体的量存在下成熟，d）引发和扩增存在于 样品和e）分析引发T细胞的功能。