公开(公告)号：US20160265057A1

公开(公告)日：2016-09-15

申请号：US15025207

申请日：2014-09-25

申请人： INSTITUTE FOR SYSTEMS BIOLOGY

发明人： Jennifer Joy SMITH ,  Samuel Anthony DANZIGER ,  John David AITCHISON ,  Leslie Rae MILLER

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/158 ,  G01N33/6896 ,  G01N2800/28 ,  G01N2800/2835 ,  G01N2800/285 ,  G01N2800/50

摘要： Methods to detect amyotrophic lateral sclerosis (ALS) or presymptomatic Alzheimer's disease (PSAD) using an indicator cell assay platform (iCAP) in a test subject are described. Specifically, the disclosure provides a method comprising contacting a biological fluid of said test subject with indicator cells and assessing said indicator cells for the level of expression of an exon of CKIgamma2 that encodes the C-terminal palmitoylated region of said CKIgamma2, to determine the probability that a test subject is afflicted with amyotrophic lateral sclerosis (ALS). Further disclosed are methods of using indicator cells that are pan neuronal populations of glutamatergic (and/or GABAergic) neurons to determine the probability of the presence of presymptomatic or symptomatic Alzheimer's disease (PSAD) in a test subject.

摘要翻译： 描述了在测试对象中使用指示剂细胞测定平台（iCAP）检测肌萎缩性侧索硬化（ALS）或症状性早老性痴呆（PSAD）的方法。 具体地，本公开提供了一种方法，包括使所述测试对象的生物流体与指示细胞接触，并评估所述指示细胞的CKIgamma2的外显子的表达水平，所述外显子编码所述CKIgamma2的C末端棕榈酰化区域，以确定概率 测试受试者患有肌萎缩性侧索硬化（ALS）。 进一步公开的是使用谷氨酸能（和/或GABA能）神经元的泛神经元群体的指示细胞来确定测试受试者中出现症状性症状或症状性阿尔茨海默氏病（PSAD）的可能性的方法。

公开(公告)号：US20160370354A1

公开(公告)日：2016-12-22

申请号：US15252039

申请日：2016-08-30

申请人： Institute for Systems Biology

发明人： Jennifer Joy SMITH ,  John David AITCHISON ,  Leslie Rae MILLER

IPC分类号： G01N33/50 ,  C12Q1/68

CPC分类号： G01N33/5091 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2800/28 ,  G01N2800/2821 ,  G01N2800/2835

摘要： The invention provides a method to detect an abnormal condition or disease state by assessing a characteristic response pattern of responder cells to indicators contained in a biological fluid of a subject. A characteristic disease-associated pattern can be obtained by determining the response pattern of responder cells to that of bodily fluids or fractions thereof from subjects known to exhibit a particular abnormal condition optionally comparing said pattern to such pattern elicited by fluids or fractions from normal subjects and identifying elements that differ. The identified elements constitute a characteristic or differential pattern which can then be used to identify the presence or stage of a disease in a test subject.

摘要翻译： 本发明提供了一种通过将受试者细胞的特征反应模式评估为包含在受试者的生物流体中的指标来检测异常状况或疾病状态的方法。 可以通过从已知显示特定异常状况的受试者确定应答细胞的体液或其部分的反应模式的响应模式，可选地将所述模式与由正常受试者的流体或分数引起的模式进行比较来获得特征性疾病相关模式， 识别不同的元素。 识别的元件构成特征或差异图案，然后可以用于鉴定测试对象中疾病的存在或阶段。