公开(公告)号：US20240280557A1

公开(公告)日：2024-08-22

申请号：US18552532

申请日：2022-03-10

Applicant: Illumina, Inc.

Inventor： Lisa Savagian ,  Burton Simpson ,  Sang Park ,  Boyan Boyanov ,  Jeffrey G. Mandell ,  Seth M. McDonald

IPC: G01N33/487 ,  B82B1/00 ,  B82B3/00 ,  C12Q1/6869

CPC classification number: G01N33/48721 ,  B82B1/008 ,  B82B3/0038 ,  C12Q1/6869

Abstract: Polypeptide nanopores synthetically functionalized with positively charged species, and methods of making and using the same, are provided herein. In some examples, a polypeptide nanopore includes a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue. The mutated amino acid residue may be synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel.