公开(公告)号：US20140287415A1

公开(公告)日：2014-09-25

申请号：US14137434

申请日：2013-12-20

Applicant: Illumina, Inc.

Inventor： Geoffrey Paul Smith ,  Roberto Rigatti ,  Tobias William Barr Ost ,  Shankar Balasubramanian ,  Raquel Maria Sanches-Kuiper

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.

Abstract translation: 修饰的DNA聚合酶对DNA具有亲和力，使得聚合酶具有在每个反应循环中将一个或多个核苷酸并入到多个单独的DNA模板中的能力。 聚合酶能够在每个反应循环中形成增加数量的生产性聚合酶-DNA复合物。 修饰的聚合酶可用于许多DNA测序应用，特别是在簇阵列的上下文中。