公开(公告)号：US4418691A

公开(公告)日：1983-12-06

申请号：US315234

申请日：1981-10-26

申请人： Ioannis V. Yannas ,  John F. Burke ,  Dennis P. Orgill ,  Eugene M. Skrabut

发明人： Ioannis V. Yannas ,  John F. Burke ,  Dennis P. Orgill ,  Eugene M. Skrabut

IPC分类号： A61K35/36 ,  A61K31/715 ,  A61K38/00 ,  A61L15/00 ,  A61L15/64 ,  A61L26/00 ,  A61L27/60 ,  A61P17/00

CPC分类号： A61L27/60 ,  Y10S623/915

摘要： This invention comprises the use of centrifugal force to introduce viable cells into a fibrous lattice, as well as fibrous lattices that are seeded with cells by the use of centrifugal force. A variety of fibrous lattices may be seeded by the methods of this invention, such as a highly porous lattice comprising collagen fibers crosslinked with glycosaminoglycan. Before the centrifugation, a piece of intact tissue is harvested from a donor site. It is treated with one or more substances, such as trypsin or collagenase, to dissociate cells from the tissue. The cells are then mixed with an aqueous solution to create an aqueous suspension of cells. A piece of fibrous lattice is placed within a container, referred to herein as a "bucket," that is suitable for rotation by a centrifuge. The aqueous suspension of cells is placed within the bucket, in contact with the lattice. The centrifuge is then rotated. Centrifugal force causes the cells, which are denser than the aqueous solution, to be forced into the lattice in a relatively uniform distribution. By controlling various parameters, cells can be seeded into any desired location within a lattice.

摘要翻译： 本发明包括使用离心力将活细胞引入纤维网格，以及通过使用离心力与细胞接种的纤维网格。 各种纤维网格可以通过本发明的方法接种，例如包含与糖胺聚糖交联的胶原纤维的高度多孔的网格。 在离心之前，从供体部位收获一块完整的组织。 用一种或多种物质（例如胰蛋白酶或胶原酶）处理以从组织中分离细胞。 然后将细胞与水溶液混合以产生细胞的水悬浮液。 将一片纤维格子放置在适合于离心机旋转的容器内，这里称为“桶”。 将细胞的水悬浮液置于桶内，与晶格接触。 然后旋转离心机。 离心力使得比水溶液更致密的细胞以相对均匀的分布被迫进入晶格。 通过控制各种参数，可将细胞接种到晶格内的任何所需位置。