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公开(公告)号：US10030238B2
公开(公告)日：2018-07-24
申请号：US14427234
申请日：2013-10-31
Applicant: Ipsen Bioinnovation Ltd.
Inventor： Aimee Cossins , Matthew Beard , Philip Marks
Abstract: The invention provides a nucleic acid sequence comprising a sequence of contiguous nucleotides, wherein said sequence of contiguous nucleotides has at least 90% sequence identity to the nucleic acid sequence of SEQ ID NO: 1, and wherein said sequence of contiguous nucleotides encodes a single-chain BoNT/E1 protein. The present invention also provides methods for producing soluble single-chain BoNT/E1 protein in an E. coli host cell, together with methods for producing soluble di-chain BoNT/E1 protein.
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