公开(公告)号：US20100272651A1

公开(公告)日：2010-10-28

申请号：US12764566

申请日：2010-04-21

申请人： Irene Georgakoudi ,  Derrick Hwu ,  Steven Boutrus ,  Charlotte Kuperwasser

发明人： Irene Georgakoudi ,  Derrick Hwu ,  Steven Boutrus ,  Charlotte Kuperwasser

IPC分类号： A61K49/00 ,  C12Q1/06

CPC分类号： A61K49/0097 ,  A61K49/0047 ,  G01N33/5017 ,  G01N33/5047 ,  G01N33/5088 ,  G01N33/5091 ,  G01N33/574 ,  G01N33/582 ,  G01N2800/50 ,  G01N2800/54 ,  G01N2800/56

摘要： The present invention generally provides methods for assessing the potential of tumor formation and/or metastasis using a combination (e.g., a ratio) of the number of circulating tumor cells and the number of circulating cells exhibiting autofluorescence within a selected wavelength region (e.g., red autofluorescence). In one aspect, it is directed to a method for providing likelihood of occurrence of a primary and/or a metastatic cancerous tumor in an animal, which comprises inoculating the animal with a plurality of cancer cells, determining a ratio of a number of cancer cells relative to a number of circulating indicator cells (e.g., immature leukocytes) that exhibit autofluorescence in the inoculated animal's blood and correlating the ratio to a likelihood that the animal will develop at least one primary and/or metastatic cancerous tumor, e.g., by way of assigning a probability for tumor development and/or metastasis based on the measured ratio. The method can also be utilized in human studies using, e.g., contrast agents to identify the circulating tumor cells.

摘要翻译： 本发明通常提供使用组合（例如，比例）循环肿瘤细胞数量和在所选波长区域内显示自发荧光的循环细胞数量（例如红色）来评估肿瘤形成和/或转移的潜力的方法 自发荧光）。 一方面，涉及提供在动物中发生原发性和/或转移性癌性肿瘤的可能性的方法，其包括用多种癌细胞接种动物，确定多种癌细胞的比例 相对于在接种的动物血液中显示自发荧光的多个循环指示细胞（例如未成熟白细胞），并将该比例与动物将发展至少一个原发性和/或转移性癌性肿瘤的可能性相关联，例如通过 基于测量的比例分配肿瘤发展和/或转移的概率。 该方法还可以用于使用例如造影剂的人类研究中鉴定循环肿瘤细胞。