公开(公告)号：US20210340548A1

公开(公告)日：2021-11-04

申请号：US17271037

申请日：2019-08-30

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Itaru IMAYOSHI ,  Mayumi YAMADA ,  Yusuke SUZUKI ,  Shinji NAGASAKI

IPC: C12N15/62 ,  C12N15/63 ,  C07K14/47

Abstract: The present invention provides a photoactivatable Tet-OFF/ON system that can precisely control temporal and spatial gene expression. The present invention is a PA-Tet-OFF/ON system that includes a target gene expression cassette including a TRE having a TetO sequence, a promoter which is controlled by the TRE, and a target gene whose expression is controlled by the promoter; a first fusion protein expression cassette containing a gene which encodes a first fusion protein containing TetR or rTetR and a first protein; and a second fusion protein expression cassette containing a gene which encodes a second fusion protein containing p65AD and a second protein, in which the first protein and the second protein bind to each other to form a heterodimer only in a state of being irradiated with light at a specific wavelength.