公开(公告)号：US20210278323A1

公开(公告)日：2021-09-09

申请号：US16496532

申请日：2018-03-28

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroyuki NOJI ,  Takao ONO ,  Yoshihiro MINAGAWA

IPC: G01N1/36 ,  G01N21/78 ,  G01N21/64 ,  G01N33/569 ,  G01N33/58 ,  B01L3/00

Abstract: There is provided a method of detecting a microscopic body stored in a plurality of receptacles formed separately from each other. The method, which is provided as a technique for enclosing a to-be-detected substance such as nucleic acid, protein, virus, and cell by means of a simple operation in droplets of an extremely small volume and enabling highly sensitive detection, includes the steps of (1) introducing a solvent into a space between a lower layer part in which the receptacles are formed and an upper layer part facing a surface of the lower layer part in which surface the receptacles are formed, wherein the solvent contains the microscopic body; (2) introducing gas into the space to form a droplet of the solvent in the receptacles, wherein the droplet contains the microscopic body; and (3) detecting the microscopic body present in the droplet optically, electrically, and/or magnetically.