公开(公告)号：US20170212982A1

公开(公告)日：2017-07-27

申请号：US15321414

申请日：2015-06-24

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takeshi Murata ,  Masahiro KINOSHITA ,  Satoshi YASUDA ,  Yuuki TAKAMUKU ,  Kenji MIZUTANI ,  Nanao SUZUKI ,  Yuta KAJIWARA

IPC: G06F19/18 ,  G06F19/16

CPC classification number: G16B20/00 ,  C07K1/107 ,  C12M1/00 ,  C12M1/34 ,  G16B15/00

Abstract: The present disclosure includes predicting a three-dimensional structure of one residue mutant of a membrane protein where respective amino acid residues have been substituted by all amino acids, calculating a solvation entropy change in formation of a tertiary structure from a primary structure or formation of the tertiary structure from secondary-structure units within a transmembrane segment, and extracting a candidate of an amino acid mutant to be thermostabilized based on a difference between a solvation entropy change in the membrane protein and a solvation entropy change in the amino acid mutant.