METHOD OF MAPPING OF mRNA DISTRIBUTION WITH ATOMIC FORCE MICROSCOPY
    1.
    发明申请
    METHOD OF MAPPING OF mRNA DISTRIBUTION WITH ATOMIC FORCE MICROSCOPY 审中-公开
    用原子力显微镜分析mRNA分布的方法

    公开(公告)号:US20090258367A1

    公开(公告)日:2009-10-15

    申请号:US12416033

    申请日:2009-03-31

    IPC分类号: C12Q1/68

    摘要: The present invention relates to a method of mapping of mRNA distribution, comprising the steps of a preparing a probe DNA attached to a apical liner region of the dendron on AFM cantilever where the probe DNA can specifically hybridize a target mRNA and measuring specific adhesive force between the probe DNA and the target mRNA on sectioned tissue at nanometer resolution.

    摘要翻译: 本发明涉及一种映射mRNA分布的方法,包括以下步骤:制备连接到AFM悬臂上的树突的顶端衬里区域上的探针DNA,其中探针DNA可以特异性地杂交目标mRNA并测量其间的特异性粘附力 探针DNA和靶mRNA在纳米分辨率的切片组织上。