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1.
公开(公告)号:US20090258367A1
公开(公告)日:2009-10-15
申请号:US12416033
申请日:2009-03-31
申请人: JOON WON PARK , YU JIN JUNG , YU SHIN PARK , HONG GIL NAM
发明人: JOON WON PARK , YU JIN JUNG , YU SHIN PARK , HONG GIL NAM
IPC分类号: C12Q1/68
CPC分类号: G01Q60/42 , C12Q1/6841 , C12Q2565/601 , C12Q2565/507
摘要: The present invention relates to a method of mapping of mRNA distribution, comprising the steps of a preparing a probe DNA attached to a apical liner region of the dendron on AFM cantilever where the probe DNA can specifically hybridize a target mRNA and measuring specific adhesive force between the probe DNA and the target mRNA on sectioned tissue at nanometer resolution.
摘要翻译: 本发明涉及一种映射mRNA分布的方法,包括以下步骤:制备连接到AFM悬臂上的树突的顶端衬里区域上的探针DNA,其中探针DNA可以特异性地杂交目标mRNA并测量其间的特异性粘附力 探针DNA和靶mRNA在纳米分辨率的切片组织上。