公开(公告)号：US07563621B2

公开(公告)日：2009-07-21

申请号：US10506098

申请日：2003-02-17

Applicant: James A Profitt ,  Alexander H. Orn

Inventor： James A Profitt ,  Alexander H. Orn

IPC: G01N33/48

CPC classification number: G01N33/54353 ,  G01N33/52

Abstract: A method of applying an organic acid reagent having limited transolubility in organic solvents, aqueous solvents, and mixtures thereof to a component of a diagnostic test device by mixing an organic acid reagent and an amine to form a salt complex, dissolving the salt complex in a solvent to release the organic acid reagent into the solvent, and applying the solvent to the component of the diagnostic test device such that the organic acid reagent present in the solvent becomes integrated into the diagnostic test device. The amine is represented by the formula HmNRn wherein m is 0, 1, or 2; n is 1, 2, or 3; the sum of m and n is 3; and R is an independently selected group which renders the salt complex soluble in aqueous solvents, organic solvents, or mixtures thereof. The amine is also represented by the formula NR4+ wherein R is an independently selected group which renders the salt complex soluble in aqueous solvents, organic solvents, or mixtures thereof. The organic acid reagent is selected from a carboxylic acid, a sulfonic acid, a phosphoric acid, and mixtures thereof. Preferred reagents are dyes like pyragallol red. In a specific embodiment the diagnostic test device is a paper test strip.

Abstract translation: 一种通过混合有机酸试剂和胺形成盐络合物，将有机溶剂在有机溶剂，含水溶剂及其混合物中的有机酸试剂施用于诊断试验装置的组分的方法，将盐络合物溶解在 溶剂将有机酸试剂释放到溶剂中，并将溶剂施加到诊断测试装置的组分，使得存在于溶剂中的有机酸试剂整合到诊断测试装置中。 胺由式HmNRn表示，其中m为0,1或2; n为1,2或3; m和n的和为3; 并且R是独立选择的基团，其使盐络合物可溶于含水溶剂，有机溶剂或其混合物中。 胺也由式NR4 +表示，其中R是独立选择的基团，其使盐络合物可溶于含水溶剂，有机溶剂或其混合物。 有机酸试剂选自羧酸，磺酸，磷酸及其混合物。 优选的试剂是如吡咯咯醇红的染料。 在具体实施例中，诊断测试装置是纸张测试条。