Apparatus for and method of measuring flourescence lifetime
    2.
    发明申请
    Apparatus for and method of measuring flourescence lifetime 审中-公开
    仪器和测量荧光寿命的方法

    公开(公告)号:US20070057198A1

    公开(公告)日:2007-03-15

    申请号:US10564342

    申请日:2004-07-15

    IPC分类号: G01N21/64

    CPC分类号: G01N21/6408 G07D7/1205

    摘要: A method of measuring fluorescence lifetime includes illuminating a sample containing at least one fluorophore with light to excite fluorescence and switching the intensity of the excitation light repeatedly between a first intensity I1 and a second intensity 12. Emitted light caused by fluorescence of the sample is detected and a detected light signal is generated. The detected light signal is switched repeatedly to divide it into first and second portions, and the amount of light detected during each of the first and second portions is measured to obtain a first emitted light value S1 and a second emitted light value S2. The fluorescence lifetime is determined from the first and second emitted light values S1 and S2.

    摘要翻译: 测量荧光寿命的方法包括用光照射含有至少一个荧光团的样品以激发荧光,并在第一强度I 1和第二强度1之间重复切换激发光的强度。 2 。 检测由样品的荧光引起的发光,并产生检测到的光信号。 检测到的光信号被重复地切换以将其分成第一和第二部分,并且测量在第一和第二部分的每一个期间检测到的光量,以获得第一发射光值S 1和 第二发射光值S 2。 从第一和第二发射光值S 1和S 2确定荧光寿命。