公开(公告)号：US20230149464A1

公开(公告)日：2023-05-18

申请号：US17988910

申请日：2022-11-17

Applicant: Janssen Biotech, Inc.

Inventor： Andrew W. SNOWDEN ,  Ali ZAMANI

IPC: A61K35/17 ,  C12N5/0783 ,  A61P35/00

CPC classification number: A61K35/17 ,  C12N5/0646 ,  A61P35/00 ,  C12N2501/998 ,  C12N2533/20 ,  C12N2501/599 ,  C12N2501/2302 ,  C12N2501/2312 ,  C12N2501/2315 ,  C12N2501/2318 ,  C12N2501/2321

Abstract: The present disclosure is directed to a feeder-cell free methods of producing an expanded natural killer (NK) cell preparation. This method comprises providing a starting preparation of NK cells and treating the starting preparation with a natural killer cell p30-related protein (NKp30) modulating agent alone or with other expansion agents as described herein. The method further involves culturing the treated preparation under conditions effective to expand the starting preparation of NK cells to produce an expanded NK cell preparation. Other aspects of the disclosure related to therapeutic preparations of NK cells produced in accordance with the methods described herein.