公开(公告)号：US20220090140A1

公开(公告)日：2022-03-24

申请号：US17595160

申请日：2020-04-21

申请人： Janssen Biotech, Inc.

发明人： Hsu-Feng Ko ,  Ravinder Bhatia ,  Soumya Mohanan Krishnathu ,  Vaishali Yannone ,  Jeffrey Edward Landau ,  Bas Diepenbroek ,  Guus Bjorn Erkens ,  Elisabeth Meulenbroek ,  Feras Nachmi Alazi

IPC分类号： C12N15/86

摘要： A method for purifying a viral vector from a solution including the viral vector and host cell proteins (HCP) is provided. The method includes circulating the solution across an ultrafiltration/diafiltration membrane using tangential flow filtration (TFF) mode with continuous addition of diafiltration buffer. The method further includes filtering the solution across the ultrafiltration/diafiltration membrane to provide a permeate and a retentate. The retentate is collected such that a purified viral vector solution is obtained and retained in the retentate. A volume of the retentate is kept constant by the continuous addition of diafiltration buffer and the HCP is filtered out via the permeate.