公开(公告)号：US20250163098A1

公开(公告)日：2025-05-22

申请号：US18696694

申请日：2022-09-27

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Masahito Inagaki ,  Fumitaka Hashiya ,  Haruka Hiraoka ,  Naoko Abe

IPC: C07H21/04

Abstract: There is provided a nucleic acid strand cleaving method including a nucleic acid preparation step of preparing a nucleic acid to be cleaved having a structure represented by the following Formula (1), and a cleaving step of reacting the nucleic acid to be cleaved with a cleaving agent to cleave the nucleic acid to be cleaved at the part X of the Formula (1) to generate a nucleic acid having a structure represented by the following Formula (2). The cleaving agent is a metal nanoparticle containing an atom selected from the group consisting of silver, mercury, and cadmium. [Chemical Formula 1] Here, B represents a base, and X represents sulfur or selenium. NucA is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 5′ end side with reference to the X. NucB is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 3′ end side with reference to the X.