公开(公告)号：US20040259257A1

公开(公告)日：2004-12-23

申请号：US10780739

申请日：2004-02-19

申请人： Japan Tobacco, Inc.

发明人： Shoichi Suzuki

IPC分类号： C12N015/82 ,  C07H021/04 ,  C12N015/87 ,  C12N015/09 ,  C12N015/63

CPC分类号： C12N15/8223 ,  C07K14/415 ,  C12N9/88

摘要： A novel nucleic acid which can exert promoter activity in phloem tissue as well as a transformed plant containing the nucleic acid and that can express a desired structural gene controlled by the nucleic acid in its phloem tissue is disclosed. The nucleic acid of the present invention has the nucleotide sequence shown in SEQ ID NO: 1 or 2 in SEQUENCE LISTING or a nucleic acid having a nucleotide sequence which is the same as the nucleotide sequence shown in SEQ ID NO: 1 or 2 in SEQUENCE LISTING except that one or a plurality of nucleotides are substituted, deleted, inserted or added, the latter nucleic acid having a promoter activity in phloem tissue of a plant, or a nucleic acid which is a part of anyone of the nucleic acids, that has a promoter activity in phloem tissue of a plant. The transformed plant of the present invention is one transformed with the nucleic acid of the present invention and a desired structural gene which is functionally ligated to a site downstream of the nucleic acid and which is controlled by the nucleic acid as a promoter, which transformed plant expresses the structural gene in its phloem tissue.

摘要翻译： 公开了一种可以在韧皮组织中发挥启动子活性的新型核酸，以及含有该核酸并能在其韧皮组织中表达由该核酸控制的所需结构基因的转化植物。 本发明的核酸具有SEQ ID NO：1或SEQ ID NO：1或SEQ ID NO：1或SEQ ID NO：1或SEQ ID NO：1或SEQ ID NO：1或SEQ ID NO：1所示核苷酸序列的核苷酸序列的核苷酸序列 列出，除了一个或多个核苷酸被取代，缺失，插入或添加，后一核酸在植物的韧皮组织中具有启动子活性，或作为任何核酸的一部分的核酸，其具有 植物韧皮组织中的启动子活性。 本发明的转化植物是用本发明的核酸转化的所需的结构基因，其功能上连接到核酸下游位点并由核酸作为启动子控制，所述转化植物 在其韧皮组织中表达结构基因。

公开(公告)号：US20030221219A1

公开(公告)日：2003-11-27

申请号：US10368396

申请日：2003-02-20

申请人： Japan Tobacco Inc.

发明人： Masao Arai ,  Shoichi Suzuki ,  Nobuhiko Murai ,  Shigehiro Yamada ,  Shozo Ohta ,  James N. Burnell

IPC分类号： A01H001/00 ,  C12N015/82

CPC分类号： C12N9/88 ,  C12N15/8242 ,  C12N15/8243 ,  C12N15/8269

摘要： The present invention relates a method for transforming a C3 plant to provide it with a C4 photoshynthetic pathway by way of the introduction of some genes participating in a C4 photosynthetic pathway. To this end, the method of the invention comprises introducing a phosphoenolpyruvate carboxylase (PEPC) and a gene coding for a phosphoenolpyruvate carboxykinase (PCK) which has been connected with a DNA fragment coding for a transit peptide into a C3 plant.

摘要翻译： 本发明涉及通过引入参与C4光合途径的一些基因来转化C3植物以提供C4光合途径的方法。 为此，本发明的方法包括将已经与编码转运肽的DNA片段连接的C3磷酸烯醇丙酮酸羧化酶（PEPC）和编码磷酸烯醇丙酮酸羧激酶（PCK）的基因导入C3植物。