公开(公告)号：US20140115730A1

公开(公告)日：2014-04-24

申请号：US14006605

申请日：2012-03-20

Applicant: Jason Michael Cope

Inventor： Jason Michael Cope

IPC: A01H1/04 ,  A01H1/02 ,  G01N33/569 ,  C12Q1/68

CPC classification number: A01H1/04 ,  A01H1/02 ,  C12Q1/6895 ,  G01N33/56961

Abstract: A method for sorting pollen containing genetic elements of interest may include associating a genetic marker with seeds defining the genetic elements of interest, growing plants from the seeds, collecting pollen from the seeds, evaluating the pollen for presence of the genetic markers, and sorting the pollen based on presence or absence of the genetic markers. A method of determining viability of pollen may evaluate the optical density of grains of pollen and compare the optical density to an optical density threshold. Further, a method for identifying plants defining a genetic marker with seeds defining the genetic element of interest, growing plants from the seeds, and evaluating the plants for presence of the genetic marker. Additionally, a method for identifying a genotype of pollen may include evaluating pollen via spectral and/or hyperspectral imaging,

Abstract translation: 用于分选含有感兴趣的遗传元件的花粉的方法可以包括将遗传标记物与限定感兴趣的遗传元件的种子，从种子生长的植物，从种子收集花粉，评估花粉存在遗传标记物，以及分类 基于存在或不存在遗传标记的花粉。 确定花粉生存力的方法可以评估花粉颗粒的光密度，并将光密度与光密度阈值进行比较。 此外，一种用于鉴定用定义感兴趣的遗传元件的种子定义遗传标记的植物的方法，从种子生长植物，以及评估植物存在遗传标记。 另外，用于鉴定花粉基因型的方法可以包括通过光谱和/或高光谱成像评价花粉，

公开(公告)号：US20140223812A1

公开(公告)日：2014-08-14

申请号：US14006715

申请日：2012-03-20

Applicant: Jason Michael Cope ,  Bradley Thomas McAvoy

Inventor： Jason Michael Cope ,  Bradley Thomas McAvoy

IPC: A01H1/02

CPC classification number: A01H1/025 ,  A01H1/02

Abstract: An apparatus configured for dispensing grains of pollen may include a pollen storage container, a flow source configured to dispense the grains of pollen from the pollen storage container, and a control mechanism configured to selectively activate the flow source. The apparatus may or may not apply an electrical charge to the grains of pollen. Related methods may include steps of collecting pollen, inserting the pollen into a pollen storage container, connecting the pollen storage container to a dispensing device such as the above-described apparatus, and dispensing the pollen from the pollen storage container with the dispensing device. Known grains of pollen may be dispensed to create predetermined gamete crosses with known plants. Further, an outlet of a fan which dispenses the pollen from the pollen storage container or other parts of the dispensing device may be cleaned when swapping the pollen storage container to prevent cross-contamination.

Abstract translation: 配置用于分配花粉颗粒的装置可以包括花粉储存容器，被配置为从花粉储存容器分配花粉颗粒的流动源和被配置为选择性地激活流动源的控制机构。 该设备可以或可以不向花粉颗粒施加电荷。 相关方法可以包括收集花粉，将花粉插入花粉储存容器，将花粉储存容器连接到诸如上述装置的分配装置的步骤，以及使用分配装置从花粉储存容器分配花粉。 可以分配已知的花粉粒以产生与已知植物的预定的配子交叉。 此外，当交换花粉储存容器以防止交叉污染时，可以清洁从花粉储存容器或分配装置的其它部分分配花粉的风扇的出口。