公开(公告)号：US07787923B2

公开(公告)日：2010-08-31

申请号：US10967220

申请日：2004-10-19

申请人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amis ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman ,  Steven Keith

发明人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amis ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman ,  Steven Keith

IPC分类号： A61B5/145

CPC分类号： A61B5/14532 ,  A61B5/1455 ,  A61B5/1459 ,  A61B5/1495 ,  A61B5/4839 ,  A61B5/6848 ,  A61B2560/0223 ,  G01N21/274 ,  G01N21/7703 ,  G01N33/542 ,  G01N33/54373 ,  G01N2021/7786 ,  G01N2201/1211

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

摘要翻译： 公开了用于感测体内或体外的分析物浓度，特别是葡萄糖浓度的装置。 感测元件连接到光学导管的远端，并且包含适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，光学导管和感测元件可以容纳在插管斜面内。

公开(公告)号：US20110184259A1

公开(公告)日：2011-07-28

申请号：US12861202

申请日：2010-08-23

申请人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman ,  Steven Keith

发明人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman ,  Steven Keith

IPC分类号： A61B5/1455

CPC分类号： A61B5/14532 ,  A61B5/1455 ,  A61B5/1459 ,  A61B5/1495 ,  A61B5/4839 ,  A61B5/6848 ,  A61B2560/0223 ,  G01N21/274 ,  G01N21/7703 ,  G01N33/542 ,  G01N33/54373 ,  G01N2021/7786 ,  G01N2201/1211

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

摘要翻译： 公开了用于感测体内或体外的分析物浓度，特别是葡萄糖浓度的装置。 感测元件连接到光学导管的远端，并且包含适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，光学导管和感测元件可以容纳在插管斜面内。

公开(公告)号：US20150018641A1

公开(公告)日：2015-01-15

申请号：US13932569

申请日：2013-07-01

申请人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

发明人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

IPC分类号： A61B5/145 ,  A61B5/1459

CPC分类号： A61B5/14532 ,  A61B5/1455 ,  A61B5/1459 ,  A61B5/1495 ,  A61B5/4839 ,  A61B5/6848 ,  A61B2560/0223 ,  G01N21/274 ,  G01N21/7703 ,  G01N33/542 ,  G01N33/54373 ,  G01N2021/7786 ,  G01N2201/1211

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

摘要翻译： 公开了用于感测体内或体外的分析物浓度，特别是葡萄糖浓度的装置。 感测元件连接到光学导管的远端，并且包含适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，光学导管和感测元件可以容纳在插管斜面内。

公开(公告)号：US20080198358A1

公开(公告)日：2008-08-21

申请号：US11840069

申请日：2007-08-16

申请人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. Denuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

发明人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. Denuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

IPC分类号： G03B27/72

CPC分类号： G01N21/7703 ,  G01N33/542 ,  G01N33/54373

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. An optical conduit, preferably an optical fiber has an optical system at the proximal end of the optical conduit. A sensing element is attached to the distal end of the optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the sensing element includes reference groups with luminescence properties that are substantially unchanged by variations in the analyte concentrations.

摘要翻译： 公开了用于感测体内或体外的分析物浓度，特别是葡萄糖浓度的装置。 光导管，优选地，光导纤维在光导管的近端具有光学系统。 感测元件连接到光学导管的远端，并且包括适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，感测元件包括具有通过分析物浓度的变化基本上不变的发光特性的参考组。

公开(公告)号：US07792561B2

公开(公告)日：2010-09-07

申请号：US11840069

申请日：2007-08-16

申请人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. Denuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

发明人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. Denuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

IPC分类号： A61B5/145

CPC分类号： G01N21/7703 ,  G01N33/542 ,  G01N33/54373

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. An optical conduit, preferably an optical fiber has an optical system at the proximal end of the optical conduit. A sensing element is attached to the distal end of the optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the sensing element includes reference groups with luminescence properties that are substantially unchanged by variations in the analyte concentrations.

摘要翻译： 公开了用于感测体内或体外的分析物浓度，特别是葡萄糖浓度的装置。 光导管，优选地，光导纤维在光导管的近端具有光学系统。 感测元件连接到光学导管的远端，并且包括适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，感测元件包括具有通过分析物浓度的变化基本上不变的发光特性的参考组。

公开(公告)号：US07496392B2

公开(公告)日：2009-02-24

申请号：US10721797

申请日：2003-11-26

申请人： Javier Alarcón ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

发明人： Javier Alarcón ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

IPC分类号： A61B5/145

CPC分类号： G01N21/7703 ,  G01N33/542 ,  G01N33/54373

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is described. An optical conduit, preferably an optical fiber, has an optical system at the proximal end of the optical conduit. A sensing element is attached to the distal end of the optical conduit, and may include at least one binding protein adapted to bind with at least one target analyte. The sensing element further may also include at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the sensing element includes reference groups with luminescence properties that are substantially unchanged by variations in the analyte concentrations.

摘要翻译： 描述了用于感测体内或体外分析物浓度，特别是葡萄糖浓度的装置。 光导管，优选光纤，在光导管的近端具有光学系统。 感测元件连接到光学导管的远端，并且可以包括适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还可以包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，感测元件包括具有通过分析物浓度的变化基本上不变的发光特性的参考组。

公开(公告)号：US07629172B2

公开(公告)日：2009-12-08

申请号：US10949557

申请日：2004-09-27

申请人： Javier Alarcon ,  Christopher J. Knors ,  J. Bruce Pitner

发明人： Javier Alarcon ,  Christopher J. Knors ,  J. Bruce Pitner

IPC分类号： G01N33/48

CPC分类号： C07K17/04 ,  G01N33/66 ,  Y10S435/817 ,  Y10T436/144444

摘要： The invention is directed to entrapped mutated binding proteins, mutated binding proteins containing reporter groups, compositions of mutated binding proteins containing reporter groups in analyte permeable matrixes, and their use as analyte biosensors both in vitro and in vivo.

摘要翻译： 本发明涉及包埋的突变结合蛋白，含有报道基团的突变结合蛋白，在分析物可渗透基质中含有报告基团的突变结合蛋白的组合物，以及它们在体外和体内的分析物生物传感器的用途。

公开(公告)号：US07767821B2

公开(公告)日：2010-08-03

申请号：US12489078

申请日：2009-06-22

申请人： J. Bruce Pitner ,  Douglas B. Sherman ,  Joseph Thomas

发明人： J. Bruce Pitner ,  Douglas B. Sherman ,  Joseph Thomas

IPC分类号： C07D413/06 ,  A61K31/538 ,  G01N33/48

CPC分类号： C09B23/10 ,  C07D207/02 ,  C09B23/105 ,  G01N33/582 ,  G01N33/66

摘要： Reactive fluorescent dyes compositions and methods of using same are disclosed. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed having thiol-reactive groups. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed that exhibit a fluorescence emission of at least about 575 nm. Biosensors are disclosed having a binding protein and a squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus.

摘要翻译： 公开了活性荧光染料组合物及其使用方法。 揭示了具有硫醇反应性基团的方钠核，尼罗红核，苯并恶唑核，香豆素核或氮杂香豆素核染料。 披露了方解子核，尼罗红核，苯并恶唑核，香豆素核或者aza香豆素核染料，其表现出至少约575nm的荧光发射。 公开了具有结合蛋白和方酸核，尼罗红核，苯并恶唑核，香豆素核或者aza香豆素核的生物传感器。

公开(公告)号：US20100003763A1

公开(公告)日：2010-01-07

申请号：US12489121

申请日：2009-06-22

申请人： J. Bruce Pitner ,  Douglas B. Sherman ,  Arounaguiry Ambroise ,  K. Joseph Thomas

发明人： J. Bruce Pitner ,  Douglas B. Sherman ,  Arounaguiry Ambroise ,  K. Joseph Thomas

IPC分类号： G01N21/76 ,  C07D265/34

CPC分类号： C09B23/10 ,  C07D207/02 ,  C09B23/105 ,  G01N33/582 ,  G01N33/66

摘要： Reactive fluorescent dyes compositions and methods of using same are disclosed. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed having thiol-reactive groups. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed that exhibit a fluorescence emission of at least about 575 nm. Biosensors are disclosed having a binding protein and a squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus.

摘要翻译： 公开了活性荧光染料组合物及其使用方法。 揭示了具有硫醇反应性基团的方钠核，尼罗红核，苯并恶唑核，香豆素核或氮杂香豆素核染料。 披露了方解子核，尼罗红核，苯并恶唑核，香豆素核或者aza香豆素核染料，其表现出至少约575nm的荧光发射。 公开了具有结合蛋白和方酸核，尼罗红核，苯并恶唑核，香豆素核或者aza香豆素核的生物传感器。

公开(公告)号：US07563891B2

公开(公告)日：2009-07-21

申请号：US11131283

申请日：2005-05-18

申请人： J. Bruce Pitner ,  Douglas B. Sherman ,  Arounaguiry Ambroise ,  Joseph Thomas

发明人： J. Bruce Pitner ,  Douglas B. Sherman ,  Arounaguiry Ambroise ,  Joseph Thomas

IPC分类号： C07D413/06 ,  A61K31/538 ,  G01N33/48

CPC分类号： C09B23/10 ,  C07D207/02 ,  C09B23/105 ,  G01N33/582 ,  G01N33/66

摘要： Reactive fluorescent dyes compositions and methods of using same are disclosed. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed having thiol-reactive groups. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed that exhibit a fluorescence emission of at least about 575 nm. Biosensors are disclosed having a binding protein and a squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus.

摘要翻译： 公开了活性荧光染料组合物及其使用方法。 揭示了具有硫醇反应性基团的方钠核，尼罗红核，苯并恶唑核，香豆素核或氮杂香豆素核染料。 披露了方解子核，尼罗红核，苯并恶唑核，香豆素核或者aza香豆素核染料，其表现出至少约575nm的荧光发射。 公开了具有结合蛋白和方酸核，尼罗红核，苯并恶唑核，香豆素核或者aza香豆素核的生物传感器。