公开(公告)号：US20110008784A1

公开(公告)日：2011-01-13

申请号：US12812489

申请日：2009-01-12

申请人： Jeannie T. Lee ,  Li-Feng Zhang

发明人： Jeannie T. Lee ,  Li-Feng Zhang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q2600/158

摘要： Disclosed herein are methods and compositions for the identification of teloRNA marks to assess the differentiation status of an individual stem cell or a population of stem cells, to diagnose whether and to what extent a stem cell or stem cell culture has already initiated cell differentiation, and to monitor the differentiation status of an individual stem cell or a stem cell culture during passage. The use of these methods and compositions to monitor the pluripotency and differentiation status of a stem cell or stem cell culture during differentiation enables the identification of undifferentiated and pluripotent stem cells prior to the initiation of differentiation. The methods and compositions can also be used to assess and maintain cell viability; to identify cells or a population of cells that are in a state of poor cell health; and to reduce cell growth or treat a diseased cell including, for example, pre-cancerous cells, cancerous cells, apoptotic cells, aging cells, cells undergoing stress, and otherwise diseased or dysfunctional cells.

摘要翻译： 本文公开了用于鉴定端粒RNA标记的方法和组合物，以评估个体干细胞或干细胞群体的分化状态，以诊断干细胞或干细胞培养物是否以及在多大程度上已经引发细胞分化，以及 监测通过过程中个体干细胞或干细胞培养物的分化状态。 这些方法和组合物在分化过程中监测干细胞或干细胞培养物的多能性和分化状态的使用使得能够在分化开始之前鉴定未分化和多能干细胞。 方法和组合物也可用于评估和维持细胞活力; 识别处于细胞健康状况不佳的细胞或细胞群体; 并且减少细胞生长或治疗包括例如癌前细胞，癌细胞，凋亡细胞，衰老细胞，经历应激的细胞，以及否则患病或功能失调的细胞的病变细胞。

公开(公告)号：US09528154B2

公开(公告)日：2016-12-27

申请号：US12812489

申请日：2009-01-12

申请人： Jeannie T. Lee ,  Li-Feng Zhang

发明人： Jeannie T. Lee ,  Li-Feng Zhang

IPC分类号： C12Q1/68 ,  C12N5/0735

CPC分类号： C12Q1/6881 ,  C12Q2600/158

摘要： Disclosed herein are methods and compositions for the identification of teloRNA marks to assess the differentiation status of an individual stem cell or a population of stem cells, to diagnose whether and to what extent a stem cell or stem cell culture has already initiated cell differentiation, and to monitor the differentiation status of an individual stem cell or a stem cell culture during passage. The use of these methods and compositions to monitor the pluripotency and differentiation status of a stem cell or stem cell culture during differentiation enables the identification of undifferentiated and pluripotent stem cells prior to the initiation of differentiation. The methods and compositions can also be used to assess and maintain cell viability; to identify cells or a population of cells that are in a state of poor cell health; and to reduce cell growth or treat a diseased cell including, for example, pre-cancerous cells, cancerous cells, apoptotic cells, aging cells, cells undergoing stress, and otherwise diseased or dysfunctional cells.

摘要翻译： 本文公开了用于鉴定端粒RNA标记的方法和组合物，以评估个体干细胞或干细胞群体的分化状态，以诊断干细胞或干细胞培养物是否以及在何种程度上已经引发细胞分化，以及 监测通过过程中个体干细胞或干细胞培养物的分化状态。 这些方法和组合物在分化过程中监测干细胞或干细胞培养物的多能性和分化状态的使用使得能够在分化开始之前鉴定未分化和多能干细胞。 方法和组合物也可用于评估和维持细胞活力; 识别处于细胞健康状况不佳的细胞或细胞群; 并且减少细胞生长或治疗包括例如癌前细胞，癌细胞，凋亡细胞，衰老细胞，经历应激的细胞，以及否则患病或功能失调的细胞的病变细胞。