摘要:
An isolated rice non-endosperm tissue expression promoter, OsTSP I, and the use thereof. The promoter comprises the defined sequence of 1785 by (SEQ ID NO: 1), given in the specification, or its fragment or variant, or a nucleotide sequence If which hybridizes to SEQ ID NO: 1, or its fragment or variant, under stringent conditions. The activity of OsTSP I is comfirmed by transgenic methods. As determined histochemically, OsTSP I reglulates GUS expression in a tissue-specific manner and is not active in endosperm tissues. The OsTSP I can be used as a powerful tool for the investigation and control of gene expression in rice and other crops. It is particularly advantageous for development of safe transgenic foods such as rice.
摘要翻译:分离的水稻非胚乳组织表达启动子OsTSP I及其用途。 启动子包括在说明书中给出的(SEQ ID NO:1)所定义的序列1785或其片段或变体,或在严格条件下与SEQ ID NO:1或其片段或变体杂交的核苷酸序列 条件。 OsTSP I的活性由转基因方法证实。 按组织学方法测定,OsTSP I以组织特异性方式调节GUS表达,并且在胚乳组织中不起作用。 OsTSP可作为水稻和其他作物基因表达调查和控制的有力工具。 特别有利于开发安全的转基因食品如水稻。