公开(公告)号：US06541247B1

公开(公告)日：2003-04-01

申请号：US09104772

申请日：1998-06-25

申请人： Jonas Frisën ,  Ann Marie Janson ,  Clas Johansson ,  Stefan Momma ,  Diana Clarke ,  Ming Zhao ,  Urban Lendahl ,  Kioumars Delfani

发明人： Jonas Frisën ,  Ann Marie Janson ,  Clas Johansson ,  Stefan Momma ,  Diana Clarke ,  Ming Zhao ,  Urban Lendahl ,  Kioumars Delfani

IPC分类号： C12N500

CPC分类号： C12N5/0623 ,  C12N2510/00

摘要： The present invention relates to a method of isolating ependymal neural CNS stem cells from a post-natal animal or a human, which method comprises the steps of (a) screening single cells obtained by dissociating CNS tissue from said animal for cells exhibiting at least one characteristic of an ependymal neural stem cell; and (b) recovering the cells that exhibit the characteristic or characteristics screened for in step (a). The screening may be performed for a specific cell surface protein or by previously labeling the ependymal cells. The invention also relates to isolated ependymal neural CNS stem cells, in vitro and in vivo assays based on the findings according to the invention and various uses of the ependymal neural stem cells according to the invention.

摘要翻译： 本发明涉及一种从产后动物或人体分离室管膜神经CNS干细胞的方法，该方法包括以下步骤：（a）筛选通过从所述动物解离CNS组织获得的细胞，所述单细胞显示至少一种 室管膜神经干细胞的特征; （b）回收表现出在步骤（a）中筛选的特征或特征的细胞。可以对特定的细胞表面蛋白进行筛选或通过预先标记室管膜细胞进行筛选。本发明还涉及分离的室管膜神经中枢神经系统干细胞 基于根据本发明的发现和根据本发明的室管膜神经干细胞的各种用途的体外和体内测定。