公开(公告)号：US20090104223A1

公开(公告)日：2009-04-23

申请号：US11870088

申请日：2007-10-10

申请人： Julio Cesar Aguilar Rubido ,  Eduardo Penton Arias ,  Dina Tleugabulova ,  Minerva Sewer Mensies ,  Verena Lucila Muzio Gonzalez ,  Gerardo Enrique Guillen Nieto ,  Iloki Assanga Simon Bernard ,  Luis Javier Cruz Ricondo ,  Viviana Falcon Cama ,  Yanet Tambara Hernandez

发明人： Julio Cesar Aguilar Rubido ,  Eduardo Penton Arias ,  Dina Tleugabulova ,  Minerva Sewer Mensies ,  Verena Lucila Muzio Gonzalez ,  Gerardo Enrique Guillen Nieto ,  Iloki Assanga Simon Bernard ,  Luis Javier Cruz Ricondo ,  Viviana Falcon Cama ,  Yanet Tambara Hernandez

IPC分类号： A61K39/385 ,  A61P37/04

CPC分类号： A61K39/292 ,  A61K39/12 ,  A61K2039/54 ,  A61K2039/543 ,  A61K2039/545 ,  A61K2039/55505 ,  A61K2039/575 ,  C12N2730/10134

摘要： The invention relates to a method for obtaining aggregated antigenic structures that are capable of enhancing an immune response to aggregate antigens administered systemically and/or mucosally generating powerful immune response and to the chemical structures resulting from the application of said method, to the formulations obtained from such structures and their use. The method describes the obtention of novel aggregate antigenic structures by using aggregating, delipidating or oxidating agents or compounds enabling the release of lipids from the particles and their heterogeneous aggregation, wherein aggregates with particle sizes of between 30 and 500 nm are subsequently selected by means of a molecular exclusion process. The aggregation state can also be provoket inside the yeast by changing incubation conditions. The resulting structures can be used conveniently adjuvated or in a formulation in which several antigens can be introduced, wherein synergism between said components is found with respect to the immunogenicity of the response obtained. The preparation may also contain stabilizers and preservatives. The resulting antigenic structures can be used in the pharmaceutical industry as preventive or therapeutic vaccine formulation both for human and veterinary use and as part of diagnostic system.

摘要翻译： 本发明涉及一种用于获得聚集的抗原结构的方法，所述聚集的抗原结构能够增强对于全身施用和/或粘膜产生强大的免疫应答的聚集抗体的免疫应答和由应用所述方法产生的化学结构与从 这种结构及其用途。 该方法描述了通过使用聚集，脱脂或氧化剂或能够从颗粒中释放脂质及其不均匀聚集的新型聚集抗原结构的获得，其中随后通过以下步骤选择粒度为30-500nm的聚集体： 分子排除过程。 聚集状态也可以通过改变孵育条件在酵母内进行。 所得到的结构可以方便地用于佐剂或在可以引入几种抗原的制剂中，其中发现所述组分之间的协同作用与获得的反应的免疫原性有关。 该制剂还可含有稳定剂和防腐剂。 所得到的抗原结构可用于制药工业，作为用于人类和兽医用途的预防或治疗疫苗制剂，并且作为诊断系统的一部分。