公开(公告)号：US20100167336A1

公开(公告)日：2010-07-01

申请号：US12676217

申请日：2008-09-04

申请人： Jung Ho Son ,  Jay Hong Whang ,  Young Seok Gang

发明人： Jung Ho Son ,  Jay Hong Whang ,  Young Seok Gang

IPC分类号： C12Q1/02

CPC分类号： C12N5/0612

摘要： Current invention is about the sex-specific separation of sperm in high purity. For more details, this invention is about the methods for improving the separation efficiency of sperm by removing factors deteriorating separation efficiency by utilizing several parameters and adjusting few conditions for the purpose of separating sperm into X-chromosome bearing and Y-chromosome bearing groups in high purity. Current invention, for the purpose of separating X-chromosome bearing and Y-chromosome bearing sperm based on physiological characteristics, utilize the difference of sperm's nucleus width as a parameter to recognize the difference in DNA content between X and Y chromosome. Current invention is about the sex-specific separation of sperm in high purity. This invention, unlike any known conventional methods, requires neither special conditions nor special treatments, can be conducted in a more realistic environment and, as a results, sex-specific sorting of sperm can be conducted without losing viability or motility. Therefore, the limitation conferred on sorting efficiency by the motility can be overcome by our invention.

摘要翻译： 本发明涉及高纯度精子的性别分离。 更多细节，本发明涉及通过利用若干参数和调整少数条件将精子分离为高染色体的X染色体和Y染色体轴承组的目的，通过去除降低分离效率的因子来提高精子分离效率的方法。 纯度。 本发明为了基于生理特征分离携带X染色体和Y染色体的精子，利用精子核宽度的差异作为参考，识别X和Y染色体之间DNA含量的差异。 本发明涉及高纯度精子的性别特异性分离。 本发明与任何已知的常规方法不同，既不需要特殊条件也不需要特殊处理，可以在更现实的环境中进行，并且作为结果，可以进行精子的性别特异性分选而不丧失活力或活力。 因此，通过我们的发明可以克服通过运动性分选效率的限制。