公开(公告)号：US20210076584A1

公开(公告)日：2021-03-18

申请号：US17106463

申请日：2020-11-30

申请人： Keygene N.V.

发明人： Rik Hubertus Martinus Op Den CAMP ,  Peter Johannes Van DIJK ,  Anthony GALLARD

IPC分类号： A01H1/08 ,  C07K14/415 ,  C12N15/82

摘要： It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.

公开(公告)号：US20190029202A1

公开(公告)日：2019-01-31

申请号：US15763710

申请日：2016-10-03

申请人： KEYGENE N.V.

发明人： Rik Hubertus Martinus OP DEN CAMP ,  Peter Johannes VAN DIJK ,  Anthony GALLARD

IPC分类号： A01H1/08 ,  C07K14/415 ,  C12N15/82

摘要： It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.

公开(公告)号：US20190225657A1

公开(公告)日：2019-07-25

申请号：US16302949

申请日：2017-05-19

申请人： Keygene N.V.

发明人： Rik Hubertus Martinus OP DEN CAMP ,  Peter Johannes VAN DIJK ,  Anthony GALLARD

IPC分类号： C07K14/415 ,  A01H6/46 ,  A01H1/08

摘要： The present disclosure provides a modified CenH3 protein that, when present in a plant, allows the plant to be used as a haploid inducer line for plant breeding purposes. Polynucleotides encoding such modified CenH3 proteins, chimeric genes and vectors comprising such polynucleotides, host cells, and plants comprising such polynucleotides, chimeric genes or vectors are also provided. Additionally, methods for making such plants as well as methods for producing haploid or doubled haploid plants using such plants are disclosed.

公开(公告)号：US20190078117A1

公开(公告)日：2019-03-14

申请号：US15763701

申请日：2016-10-03

申请人： Keygene N.V.

发明人： Rik Hubertus Martinus OP DEN CAMP ,  Peter Johannes VAN DIJK ,  Anthony GALLARD

IPC分类号： C12N15/82 ,  C07K14/415

摘要： It was found that plants comprising modified CENPC protein comprising one or more active mutations which affect the functioning of CENPC protein yet allow plants expressing said modified CENPC protein to be viable, are able to induce haploid offspring after a cross to or with a wild type plant comprising a endogenous CENPC protein. The invention relates to generation of haploid and doubled haploid plants.