公开(公告)号：US20250062038A1

公开(公告)日：2025-02-20

申请号：US18451410

申请日：2023-08-17

Applicant: KING FAHD UNIVERSITY OF PETROLEUM AND MINERALS

Inventor： Amjad Bajes KHALIL ,  Muhammad NAEEM ,  Hanoof Fahad ALKHODAIRY ,  Aasif HELAL

IPC: G16H50/80 ,  G01N33/58

Abstract: A method of detecting SARS-COV in a sample includes contacting a metal-organic framework with at least one fluorophore-labeled single-stranded probe deoxyribose nucleic acid (DNA) to form a biosensor. The metal-organic framework of the method is zeolitic imidazolate framework-8 (ZIF-8), and the fluorophore-labeled single-stranded probe DNA (p-DNA) has a fluorescence signal at 513 to 517 nm. The method further includes contacting the sample with the biosensor in a solution, and the sample comprises a target sequence of SARS-COV. The target sequence of SARS-COV and the fluorophore-labeled single-stranded p-DNA of the method hybridize to form a double-stranded product. Following the step of the hybridization forming the double-stranded product, the method includes detecting the double-stranded product by observing a change in fluorescence.