公开(公告)号：US20220213463A1

公开(公告)日：2022-07-07

申请号：US17292967

申请日：2018-11-28

申请人： KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY

发明人： Jung-Hyun LEE ,  Jae Kyu LIM ,  Hyun Sook LEE ,  Sung Gyun KANG ,  Kae Kyoung KWON ,  Yun Jae KIM ,  Ji-in YANG

IPC分类号： C12N9/96 ,  C12N9/02 ,  C12N15/52 ,  C12P7/40

摘要： The present invention relates to an Fe—S fusion protein acting as an electron transport chain, a novel carbon monoxide:formate oxidoreductase (CFOR) including the Fe—S fusion protein, novel Thermococcus strain BCF12 transformed with CFOR, and the use thereof. According to the present invention, two different enzymes may be physically linked directly to each other through the Fe—S fusion protein of the present invention, and thus electrons generated from any one enzyme may be transported directly to another enzyme through the Fe—S cluster of the Fe—S fusion protein. Accordingly, a reaction that produces a target substance with high efficiency by directly supplying electrons necessary for the production of the target substance is possible without leakage of electrons generated in any one enzyme. In addition, the present invention has an advantage in that the overall enzyme reaction rate and yield can be dramatically improved using a new electron transport reaction. Furthermore, it is possible to ensure the stability of each enzyme by allowing the enzymes to exist in a physically fixed state in cells.