公开(公告)号：US20180217164A1

公开(公告)日：2018-08-02

申请号：US15696302

申请日：2017-09-06

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo-Hyun LEE ,  Ji-Yoon KANG ,  Min-Ho KIM ,  Yun-Kyung KIM

IPC: G01N33/68 ,  G01N27/02 ,  G01N21/64 ,  G01N21/76

Abstract: According to a method for monitoring post-translational modifications of protein is provided, a first microbead by binding a protein antibody to a base bead is provided. A second microbead by binding a target protein having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first microbead, is provided. A third microbead by binding the second microbead to a first post-translational modification antibody is provided. A fourth microbead by binding the second microbead to a second post-translational modification antibody is provided. Impedances of the third and fourth microbeads are measured. A ratio of a first difference, between the impedances of the third microbead and a reference impedance, to a second difference, between the impedances of the fourth microbead and the reference impedance, is obtained.

公开(公告)号：US20200088743A1

公开(公告)日：2020-03-19

申请号：US16685432

申请日：2019-11-15

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo-Hyun LEE ,  Ji-Yoon KANG ,  Min-Ho KIM ,  Yun-Kyung KIM

IPC: G01N33/68 ,  G01N33/543 ,  G01N27/02 ,  G01N21/64

Abstract: According to a method for monitoring post-translational modifications of protein is provided, a first microbead by binding a protein antibody to a base bead is provided. A second microbead by binding a target protein having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first microbead, is provided. A third microbead by binding the second microbead to a first post-translational modification antibody is provided. A fourth microbead by binding the second microbead to a second post-translational modification antibody is provided. Impedances of the third and fourth microbeads are measured. A ratio of a first difference, between the impedances of the third microbead and a reference impedance, to a second difference, between the impedances of the fourth microbead and the reference impedance, is obtained.