公开(公告)号：US10233505B2

公开(公告)日：2019-03-19

申请号：US15543712

申请日：2016-01-15

Applicant: Kansas State University Research Foundation

Inventor： Jianfa Bai ,  Xuming Liu ,  Gary Anderson

IPC: C12Q1/689

Abstract: The present invention is concerned with PCR-based detection methods and kits for the identification, differentiation, and quantification of different bacterial strains (e.g., Gram-negative bacterial strains), and also association of two or more PCR-positive genes to a single genome. The methods generally comprise carrying out PCR reactions using at least a first PCR primer set and/or probe for at least one target nucleic acid; and a second PCR primer set and/or probe for at least a second target nucleic acid. Positive PCR reaction products are then detected to determine test samples containing positive PCR reaction products for both the first and second target nucleic acids. This information can be used to calculate the gene association rate to determine whether the sample contains, for example, Shiga toxin-producing E. coli of the O-type serogroup.

公开(公告)号：US20180010170A1

公开(公告)日：2018-01-11

申请号：US15543712

申请日：2016-01-15

Applicant: Kansas State University Research Foundation

Inventor： Jianfa Bai ,  Xuming Liu ,  Gary Anderson

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2537/143

Abstract: The present invention is concerned with PCR-based detection methods and kits for the identification, differentiation, and quantification of different bacterial strains (e.g., Gram-negative bacterial strains), and also association of two or more PCR-positive genes to a single genome. The methods generally comprise carrying out PCR reactions using at least a first PCR primer set and/or probe for at least one target nucleic acid; and a second PCR primer set and/or probe for at least a second target nucleic acid. Positive PCR reaction products are then detected to determine test samples containing positive PCR reaction products for both the first and second target nucleic acids. This information can be used to calculate the gene association rate to determine whether the sample contains, for example, Shiga toxin-producing E. coli of the O-type serogroup.