公开(公告)号：US20220356492A1

公开(公告)日：2022-11-10

申请号：US17635036

申请日：2020-08-14

Applicant: Kansas State University Research Foundation

Inventor： Kristopher Silver ,  Yoonseong Park

IPC: C12N15/88 ,  A01N63/60

Abstract: Nucleic acid delivery vehicles for delivering nucleic acid, e.g., for RNAi to cells that are typically refractory to RNAi by using extracellular vesicles (EVs) from cultured beetle cells as delivery vehicles. Instead of using high levels of long dsRNA and transfection reagents to accomplish suppression of an mRNA target in cells that don't respond to treatment with naked dsRNA, this approach applies the dsRNA to cultured beetle cells, collects nucleic-acid loaded EVs from the culture, then treats our target cells with the intracellularly loaded EVs, which results in significant enhancement of the RNAi response and greater suppression of transcript levels.

公开(公告)号：US20220338471A1

公开(公告)日：2022-10-27

申请号：US17762994

申请日：2020-09-23

Applicant: Kansas State University Research Foundation ,  The United States of America, as represented by the Secretary of Agriculture ,  Board of Regents of The University of Nebraska

Inventor： Yoonseong Park ,  Ronald Nachman ,  Troy Anderson

IPC: A01N25/32 ,  A01N25/02 ,  A01N25/12 ,  A01P7/00

Abstract: Described herein are bee-safe acaricides that target the varroa mite-specific neuropeptidergic system regulated by proctolin, and methods of use. The acaricides comprise a peptide analog or peptidomimetic of varroa mite proctolin for selective targeting of this pest in a bee colony. The compounds are also effective against other pests, such as ticks. The peptide analog or peptidomimetic generally comprises one or two amino acid residue substitutions or insertions. Also described herein are methods of screening compounds for activity on a proctolin receptor.