公开(公告)号：US20220235331A1

公开(公告)日：2022-07-28

申请号：US17603645

申请日：2020-04-17

申请人： Keio University ,  Nihon University

发明人： Hideyuki Okano ,  Seiji Shiozawa ,  Sho Yoshimatsu ,  Kazuya Edamura ,  Aozora Iguchi

IPC分类号： C12N5/074

摘要： A method of producing induced pluripotent stem cells including a step for introducing an initialization factor into somatic cells of a mammal, and culturing in a neural stem cell culture medium to obtain induced neural stem cell-like cells, and a step for cultivating said induced neural stem cell-like cells in a growth medium to obtain induced pluripotent stem cells, wherein the initialization factor contains an OCT family, a SOX family, a KLF family, a MYC family, a LIN28 family and a P53 function inhibitor.