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    Creatine amidinohydrolase from alkaligenes sp. ks-85 ferm bp-4487
    1.
    发明授权
    Creatine amidinohydrolase from alkaligenes sp. ks-85 ferm bp-4487 失效
    来自碱产生的肌酸脒基水解酶 ks-85 ferm bp-4487

    公开(公告)号：US5451520A

    公开(公告)日：1995-09-19

    申请号：US343972

    申请日：1994-11-18

    申请人： Keisuke Furukawa ,  Kyoko Hashimoto ,  Masaru Suzuki

    发明人： Keisuke Furukawa ,  Kyoko Hashimoto ,  Masaru Suzuki

    IPC分类号： C12N9/78 ,  C12N9/80 ,  C12Q1/34 ,  C12R1/05 ,  C12N1/00 ,  C12N1/12

    CPC分类号： C12Q1/34 ,  C12N9/80 ,  G01N2333/978 ,  Y10S435/829

    摘要： A creatine amidinohydrolase with the following physicochemical properties is prepared:(a) action: hydrolysis of 1 mole of creatine to form 1 mole of sarcosine and 1 mole of urea;(b) substrate specificity: specific for a creatine substrate;(c) optimum pH: 7-9;(d) optimum temperature: around 35.degree.-45.degree. C.;(e) pH stability: stable in the range of pH 5.0-10.5 at 25.degree. C. for 17 hours;(f) thermal stability: stable at a temperature up to about 45.degree. C. at pH 7.5 for 30 min.;(g) inhibitors: AgNO.sub.3, HgCl.sub.2, CuSO.sub.4, etc.; and(h) molecular weight: about 80,000.+-.5000 as determined by gel filtration.The creatine amidinohydrolase is stable in high pH range and possesses a small Km value, so that it can be purified in high pH range resulting in more easy and simple production than the conventional enzyme, and the lower Km value enables reduction in the period of time and in the amount of the enzyme for each measurement. The creatine amidinohydrolase is obtained by culturing Alkaligenes sp. KS-85 FERM BP-4487.

    摘要翻译： 制备具有以下物理化学性质的肌酸脒基水解酶：（a）作用：水解1摩尔肌酸以形成1摩尔肌氨酸和1摩尔尿素; （b）底物特异性：对肌酸底物有特异性; （c）最适pH：7-9; （d）最佳温度：约35°-45℃。 （e）pH稳定性：在25℃下在pH5.0-10.5的范围内稳定17小时; （f）热稳定性：在高达约45℃的温度下在pH7.5下稳定30分钟; （g）抑制剂：AgNO 3，HgCl 2，CuSO 4等; 和（h）通过凝胶过滤测定的分子量：约80,000 +/- 5000。 肌酸酰氨基水解酶在高pH范围内是稳定的，具有小的Km值，因此可以在高pH范围内进行纯化，从而比常规酶更容易和简单地生产，而较低的Km值可以减少一段时间 以及用于每次测量的酶的量。 肌酸酰胺水解酶通过培养阿尔卡氏菌属获得。 KS-85 FERM BP-4487。

    Catadioptric lens system capable of automatic focusing operation
    2.
    发明授权
    Catadioptric lens system capable of automatic focusing operation 失效
    反射折射透镜系统能够进行自动对焦操作

    公开(公告)号：US4989951A

    公开(公告)日：1991-02-05

    申请号：US352121

    申请日：1989-05-15

    申请人： Masaaki Miyano ,  Kyoko Hashimoto

    发明人： Masaaki Miyano ,  Kyoko Hashimoto

    IPC分类号： G02B7/09 ,  G02B7/10

    CPC分类号： G02B7/10

    摘要： A catadioptric lens system capable of an automatic focusing operation without the necessity of increasing the outer diameter of a lens barrel is provided. The catadioptric lens system comprises a focusing optical unit, a focusing optical unit holder capable of movement in a direction of an optical axis of the lens band for holding the focusing optical unit, and a driving means disposed between a mount and a reflecting mirror which is disposed to reflect light coming from an object toward an object side and connected directly or indirectly to the focusing optical unit holder for transmitting a driving force to the focusing optical unit holder at a reduced speed so as to move the focusing optical unit holder in the direction of the optical axis to effect focusing.

    摘要翻译： 提供能够进行自动聚焦操作而不需要增加透镜镜筒的外径的反折射透镜系统。 反射折射透镜系统包括聚焦光学单元，能够沿着用于保持聚焦光学单元的透镜带的光轴方向移动的聚焦光学单元保持器，以及设置在安装件和反射镜之间的驱动装置， 被配置为将来自物体的光反射到物体侧并且直接或间接地连接到聚焦光学单元保持器，用于以降低的速度将驱动力传递到聚焦光学单元保持器，以使聚焦光学单元保持器沿方向移动 的光轴进行聚焦。