公开(公告)号：US20210054393A1

公开(公告)日：2021-02-25

申请号：US17081832

申请日：2020-10-27

Applicant: Keygene N.V.

Inventor： Bernardus SCHERES ,  Renze HEIDSTRA ,  Menno Christian Cyrano PIJNENBURG ,  Michiel Theodoor Jan DE BOTH

IPC: C12N15/82 ,  C07K14/415

Abstract: The invention pertains to a method for regenerating a plant cell, preferably regenerating a shoot from a plant cell by altering the expression levels of at least WOX5 and a PLT protein, preferably WOX5 and PLT1. In addition the expression levels of further proteins can altered, such as WIND1, SHR, SCR, RBR, PLT4 and PLT5 to regenerate a shoot from a plant cell. Preferably, the expression levels are transiently altered. The invention further pertains to a nucleic acid construct suitable for transient protein expression and the use of the protein combinations for regenerating a shoot from a plant cell.