公开(公告)号：US20160251713A1

公开(公告)日：2016-09-01

申请号：US15014642

申请日：2016-02-03

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Taco Peter JESSE

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2535/138

Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.

Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。

公开(公告)号：US20180002751A1

公开(公告)日：2018-01-04

申请号：US15707650

申请日：2017-09-18

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Taco Peter JESSE ,  Adrianus Johannes VAN TUNEN

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2535/138

Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.

公开(公告)号：US20150329906A1

公开(公告)日：2015-11-19

申请号：US14808761

申请日：2015-07-24

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Adrianus Johannes VAN TUNEN ,  Antoine Antonius Arnoldus Wilhelmus JANSSEN ,  Taco Peter JESSE

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2563/179 ,  C12Q2535/122 ,  C12Q2525/191 ,  C12Q2565/514 ,  C12Q2565/601 ,  C12Q2565/631

Abstract: The invention relates to a method for the determination of a genome sequence comprising the steps of providing a physical map of a sample genome by sequencing fragment ends of pooled BAC clones; providing a set of sequence reads from a sample genome generating a contig of the physical map and the sequence reads.

Abstract translation: 本发明涉及一种用于确定基因组序列的方法，包括以下步骤：通过测序合并的BAC克隆的片段末端来提供样品基因组的物理图谱; 从样本基因组提供一组序列读取，产生物理图和序列读数的重叠群。

公开(公告)号：US20150148241A1

公开(公告)日：2015-05-28

申请号：US14613849

申请日：2015-02-04

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Taco Peter JESSE

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2535/138

Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.

Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。