公开(公告)号：US20070122909A1

公开(公告)日：2007-05-31

申请号：US10576268

申请日：2004-10-13

申请人： Koichi Nakano ,  Ayako Umetsu ,  Yuko Ooi

发明人： Koichi Nakano ,  Ayako Umetsu ,  Yuko Ooi

IPC分类号： G01N31/00

CPC分类号： G01N33/57411 ,  C12Q1/02 ,  C12Q1/37 ,  G01N33/5091 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/108331

摘要： The invention provides methods of treating cells wherein cells in a specimen containing mucus-producing cells are stabilized, mucus in the specimen is removed without affecting the cell morphology, or cells in the specimen are dispersed individually; and reagent kits to be used in said treating methods. After the cells are stabilized by treatment with a solution containing an aldehyde compound, the cell populations can be dispersed by treatment with protease. In the case of specimens containing cells aggregated with mucus, the processes of stabilizing and treating with protease may be conducted after the mucus is removed. In order to remove mucus, cysteine and/or a compound derived therefrom is preferably used.

摘要翻译： 本发明提供处理细胞的方法，其中含有产生粘液的细胞的样品中的细胞被稳定，样品中的粘液被去除而不影响细胞形态，或者样品中的细胞分散分散; 和用于所述处理方法的试剂盒。 在通过用含有醛化合物的溶液处理来稳定细胞后，通过用蛋白酶处理可以分散细胞群。 在含有与粘液聚集的细胞的样品的情况下，可以在去除粘液之后进行用蛋白酶稳定和处理的过程。 为了去除粘液，优选使用半胱氨酸和/或由其衍生的化合物。

公开(公告)号：US20070054258A1

公开(公告)日：2007-03-08

申请号：US10574471

申请日：2004-10-01

申请人： Koichi Nakano ,  Ayako Umetsu ,  Yuko Ooi

发明人： Koichi Nakano ,  Ayako Umetsu ,  Yuko Ooi

IPC分类号： A01N1/02 ,  C12N5/08

CPC分类号： A01N1/02 ,  A01N1/021 ,  G01N1/30

摘要： Mucus can be removed from cell surfaces so as to enable immunochemical labeling by bringing a cell treatment solution containing cysteine and/or a compound derived therefrom in an amount of 5 mass % or more into contact with a cell specimen containing mucus producing cells, such as uterine cervix cells. The cell preservative solution or an aqueous solution containing 30 to 60 mass % alcohol and 5 mass % or more cysteine and/or a compound derived therefrom can store cells while maintaining the state of cell dispersion without any effect on cell morphology during the period from specimen collection to analysis.

摘要翻译： 可以从细胞表面除去粘液，以便通过将含有5质量％以上的半胱氨酸和/或其衍生物的细胞处理溶液与含有粘液产生细胞的细胞样品接触来实现免疫化学标记，例如 子宫颈细胞。 细胞保存溶液或含有30〜60质量％的醇和5质量％以上的半胱氨酸和/或由其衍生的化合物的水溶液可以在保持细胞分散状态的同时存储细胞，而在样品期间对细胞形态没有任何影响 收集分析。