公开(公告)号：US20090208508A1

公开(公告)日：2009-08-20

申请号：US12094955

申请日：2006-11-24

申请人： Kong Joo Lee ,  Hyun Jung Kim ,  Jeong Woo Cho ,  Cheol Young Maeng ,  You Kyung Nam ,  Sang Mi Lim ,  Young Mee Kim

发明人： Kong Joo Lee ,  Hyun Jung Kim ,  Jeong Woo Cho ,  Cheol Young Maeng ,  You Kyung Nam ,  Sang Mi Lim ,  Young Mee Kim

IPC分类号： A61K39/395 ,  A61K31/7105 ,  C12Q1/68

CPC分类号： A61K31/70 ,  C07K14/4703 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/53 ,  C12Q1/6886 ,  C12Q2600/136

摘要： The present invention relates to a composition for diagnosing cancer metastasis comprising Ubiquitin C-terminal hydrolase-L1 (UCH-L1), a use of UCH-L1 for diagnosing cancer metastasis and a method for diagnosing cancer metastasis using the same; a composition for suppressing cancer metastasis comprising an inhibitor of UCH-L1, a use of an inhibitor of UCH-L1 for suppressing cancer metastasis and a method of suppressing cancer metastasis using the same; a composition for screening a cancer metastasis inhibitor comprising UCH-L1 protein, a use of UCH-L1 protein for screening a cancer metastasis inhibitor and a method of screening cancer metastasis inhibitor using the same; a composition for screening a cancer metastasis inhibitor comprising UCH-L1 gene, a use of UCH-L1 gene for screening a cancer metastasis inhibitor, a method of screening cancer metastasis inhibitor using the same.UCH-L1 is a key molecule to modulate cell migration including the cancer invasion according to their expressing level. Thus, the monoclonal and polyclonal antibodies and the substrate of UCH-L1 can be used for diagnosis of cancer metastasis.Also, the cancer metastasis can be suppressed by inhibiting of the expression of UCH-L1 or activity of the enzyme, thus we can screen and develop an inhibitor of UCH-L1 and use it as an adjuvant drug for anticancer therapy.

摘要翻译： 本发明涉及用于诊断癌转移的组合物，其包含泛素C末端水解酶-L1（UCH-L1），UCH-L1用于诊断癌症转移的用途和使用该组合物诊断癌症转移的方法。 用于抑制癌转移的组合物，其包含UCH-L1抑制剂，UCH-L1抑制剂用于抑制癌症转移的用途及使用其抑制癌转移的方法; 用于筛选包含UCH-L1蛋白的癌症转移抑制剂的组合物，UCH-L1蛋白用于筛选癌症转移抑制剂的方法和使用该组合物筛选癌症转移抑制剂的方法; 用于筛选包含UCH-L1基因的癌转移抑制剂的组合物，使用UCH-L1基因筛选癌症转移抑制剂，使用该组合物筛选癌症转移抑制剂的方法。 UCH-L1是根据其表达水平调节细胞迁移的关键分子，包括癌症侵袭。 因此，单克隆抗体和多克隆抗体以及UCH-L1的底物可用于癌症转移的诊断。 此外，可以通过抑制UCH-L1的表达或酶的活性来抑制癌症转移，从而可以筛选和开发UCH-L1的抑制剂，并将其用作抗癌治疗的辅助药物。