公开(公告)号：US20090318673A1

公开(公告)日：2009-12-24

申请号：US12162460

申请日：2007-01-25

申请人： Kyoko Suto ,  Hiromi Takenaka ,  Yasuhiro Takenaka

发明人： Kyoko Suto ,  Hiromi Takenaka ,  Yasuhiro Takenaka

IPC分类号： C07K1/107 ,  C07K14/435

CPC分类号： C07K14/43595

摘要： The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei, His52 in an α helix-like secondary structure: PFLLSHCMGYGFYHF (α1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength.

摘要翻译： 本发明提供了一种在保持重组表达效率的同时维持来自桡足类的GFP样荧光蛋白的荧光波长的方法，其包括鉴定用于测定蛋白质三维结构中的荧光波长的结构因子和修饰 与结构因子相关的氨基酸残基; 和通过应用所述方法获得的修饰的荧光蛋白。 例如，关于来自Chiridius poppei的GFP样荧光蛋白，包含荧光部位部位GYG的α螺旋状二级结构的His52被替换为选自以下的芳香族氨基酸：Phe， Tyr和Trp，以引起荧光峰值波长的红移; 或者被Ala，Val，Ile，Leu，Gly，Cys，Met，Ser，Thr或Asp，Asn，Glu或Gln替代，以使荧光峰值波长发生蓝移。

公开(公告)号：US08278120B2

公开(公告)日：2012-10-02

申请号：US12162460

申请日：2007-01-25

申请人： Kyoko Suto ,  Hiromi Takenaka ,  Yasuhiro Takenaka

发明人： Kyoko Suto ,  Hiromi Takenaka ,  Yasuhiro Takenaka

IPC分类号： G01N33/536 ,  G01N33/533 ,  C07K1/00 ,  C07K14/00 ,  C07K17/00

CPC分类号： C07K14/43595

摘要： The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei, His52 in an α helix-like secondary structure: PFLLSHCMGYGFYHF (α1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength.

摘要翻译： 本发明提供了一种在保持重组表达效率的同时维持来自桡足类的GFP样荧光蛋白的荧光波长的方法，其包括鉴定用于测定蛋白质三维结构中的荧光波长的结构因子和修饰 与结构因子相关的氨基酸残基; 和通过应用所述方法获得的修饰的荧光蛋白。 例如，关于来自Chiridius poppei的GFP样荧光蛋白，包含荧光部位部位GYG的α螺旋状二级结构的His52被替换为选自Phe的芳香族氨基酸， Tyr和Trp，以引起荧光峰值波长的红移; 或者被Ala，Val，Ile，Leu，Gly，Cys，Met，Ser，Thr或Asp，Asn，Glu或Gln替代，以使荧光峰值波长发生蓝移。

公开(公告)号：US5064576A

公开(公告)日：1991-11-12

申请号：US397046

申请日：1989-08-22

申请人： Kyoko Suto

发明人： Kyoko Suto

IPC分类号： A61L2/28 ,  C09D5/26 ,  C09D11/00 ,  C09K9/02 ,  G01N31/22

CPC分类号： C09K9/02 ,  A61L2/28 ,  C09D11/50 ,  C09D5/26 ,  G01N31/226 ,  C09K2211/187

摘要： A steam sensitive composition and a sterilization indicator composition which change in color only upon exposure to all of the conditions necessary for sterilization, that is, steam at a prescribed temperature for a prescribed period of time. The steam sensitive composition contains a metal complex and an exchange ligand. The metal complex is bis(dimethylglyoximato)nickel, bis(2-furyldioximato)nickel, zirconium chloranilate or bis(nioximato)nickel; and the exchange ligand is either 1) an aminocarboxylic acid which comprises from 1 to 6 carboxylic acid groups and from 1 to 4 amino groups, and its salts; or 2) citric or tartaric acids and their salts. The sterilization indicator contains as main components the steam sensitive composition described above, a binder, a solvent and preferably a color-change rate regulating component. The rate regulating components may be a cyanate or thiocyanate salt, and/or tartaric or citrate acid or their salts.