公开(公告)号：US20100086993A1

公开(公告)日：2010-04-08

申请号：US12272130

申请日：2008-11-17

申请人： LEE-JENE LAI ,  YI-HEUI HSIEH ,  SHIH-JEN LIU ,  HSIN-WEI CHEN

发明人： LEE-JENE LAI ,  YI-HEUI HSIEH ,  SHIH-JEN LIU ,  HSIN-WEI CHEN

IPC分类号： C12M1/42 ,  G01J1/42

CPC分类号： G01N21/645 ,  G01N15/1459 ,  G01N2015/1477

摘要： A cell detecting system is herein disclosed, wherein a complex is formed with a first bioactive ligand coupling to a quantum dot and recognizing and coupling to a first receptor of a cell and a second bioactive ligand coupling to a magnetic bead and recognizing and coupling to a second receptor of the cell. The magnet is configured for attracting the complex. A quantum dot measuring system configured for measuring the fluorescence of the complex includes an excitation light source, an optical system, a detecting sensor and a data capturing unit, wherein the detecting sensor includes a photomultiplier tube measuring florescence of the quantum dot excited by the excitation light source. The present invention achieves the goal of specific cell detection with high sensitivity without performing cell incubation. A quantum dot measuring system is also herein disclosed.

摘要翻译： 本文公开了一种细胞检测系统，其中复合物由与量子点偶联的第一生物活性配体形成，并且识别并偶联至细胞的第一受体和与磁珠偶联的第二生物活性配体，并识别和偶联至 细胞的第二受体。 磁体配置用于吸引复合体。 配置用于测量复合物的荧光的量子点测量系统包括激发光源，光学系统，检测传感器和数据捕获单元，其中检测传感器包括测量由激发激发的量子点的荧光的光电倍增管 光源。 本发明在不进行细胞培养的情况下实现高灵敏度的特异性细胞检测的目的。 本文还公开了量子点测量系统。