CONFOCAL MICROSCOPE AND A METHOD OF ADJUSTING A CONFOCAL MICROSCOPE

    公开(公告)号:US20240255743A1

    公开(公告)日:2024-08-01

    申请号:US18424944

    申请日:2024-01-29

    IPC分类号: G02B21/00

    摘要: A confocal microscope includes an illumination unit configured to generate an illumination light bundle, an imaging optics configured to receive detection light, a scanner configured to deflect the illumination light bundle to generate a scanning illumination and to direct the detection light into a detection beam path, a sensor disposed in the detection beam path, a pinhole diaphragm arranged in the detection beam path upstream of the sensor, an adjustable light deflector arranged in the detection beam path and configured to direct the detection light through the pinhole diaphragm onto the sensor, and a controller configured to control the scanner to adjust the confocal microscope in such a manner that the illumination light bundle is directed onto a test object, and control the light deflector in such a manner that an intensity of the detection light coming from the test object, as detected by the sensor, is optimized.

    DEVICE AND METHOD FOR FEEDING AN IMMERSION MEDIUM

    公开(公告)号:US20200319659A1

    公开(公告)日:2020-10-08

    申请号:US16835449

    申请日:2020-03-31

    IPC分类号: G05D9/12 G02B21/33

    摘要: A feed device for an immersion medium for use with an objective enabling a specimen to be imaged microscopically includes a cap fitted releasably or fixedly to the objective and delimiting a receptacle space for the immersion medium. The cap has an exit opening aligned with an optical element of the objective facing the specimen. The immersion medium held in the receptacle space is feedable through the exit opening to a target space situated between the optical element of the objective and the specimen. A sensor is integrated in the cap and has an electrode structure configured to detect an amount of the immersion medium fed through the exit opening to the target space. The electrode structure at least partly encloses the exit opening and has a spatial detection region extending away from the exit opening in a radial direction.

    MICROSCOPE AND METHOD FOR GENERATING AN OVERVIEW IMAGE OF A SAMPLE

    公开(公告)号:US20220011561A1

    公开(公告)日:2022-01-13

    申请号:US17369972

    申请日:2021-07-08

    IPC分类号: G02B21/33 G02B21/00

    摘要: A microscope includes: an optical system including an immersion objective for an immersion medium of a predetermined refractive index; an aperture stop; and a processor for setting an immersion-free imaging mode in which the optical system is operated without immersion medium. The processor controls the aperture stop in the immersion-free imaging mode to set a numerical aperture of the immersion objective to a reduced value which is lower than a nominal value of the numerical aperture, the numerical aperture being equal to the nominal value when the optical system is operated using the immersion medium without reducing the numerical aperture by the aperture stop. The processor controls the optical system in accordance with the reduced value of the numerical aperture in the immersion-free imaging mode to generate at least one image representing the overview image of the sample.

    MICROSCOPE AND METHOD FOR DETERMINING AN ABERRATION IN A MICROSCOPE

    公开(公告)号:US20210124160A1

    公开(公告)日:2021-04-29

    申请号:US17080874

    申请日:2020-10-27

    IPC分类号: G02B21/00 G02B27/00 G02B21/24

    摘要: A microscope includes an optical imaging system with an adjustable corrector, a microscope drive, a position sensitive detector, an optical measuring system and a control unit. The optical measuring system configured to form first and second measuring light beams, direct the measuring light beams into an entrance pupil of the optical imaging system eccentrically with first and second distances to the optical axis thereof, receive first and second reflection light beams, and direct the reflection light beams onto the position sensitive detector. The control unit is configured to record positions of the reflection light beams on the position sensitive detector, and determine an aberration based on the recorded positions.