公开(公告)号：US20200010810A1

公开(公告)日：2020-01-09

申请号：US16495552

申请日：2018-03-23

申请人： Lankenau Institute of Medical Research

发明人： Scott K. Dessain ,  Rama Devudu Puligedda

IPC分类号： C12N5/16 ,  C07K16/10 ,  G01N33/569 ,  C12N15/10

摘要： Compositions and methods are provided for making and using a cell having expressed on its outer plasma membrane surface an Anchor designed to form a first complex with a selected Linker. The Linker is designed to form a second complex with in a target protein that is not recognized by the Anchor. These cells can be primary cells or immortalized cells, they function as fusion partners to create hybridoma cells. The cells can be designed to bind a secreted targeted protein, e.g., a secreted antibody, to the cell surface via an immune complex formed by the Anchor-Linker and permit the identification of the existence, antigen specificity, and antigen binding affinity, titer, amount, or biological activity of the target protein. In certain embodiments, the Linker is of a species heterologous to the species of the Anchor; and the target protein is of a species heterologous to the Linker and to the Anchor.