公开(公告)号：US20210016272A1

公开(公告)日：2021-01-21

申请号：US17037826

申请日：2020-09-30

申请人： Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

发明人： Shiqi SUN ,  Huichen GUO ,  Yun ZHANG ,  Yanyan CHANG ,  Yanquan WEI ,  Jiaxi RU ,  Xiaoying ZHI ,  Ping DU ,  Xiangtao LIU ,  Hong YIN ,  Jianxun LUO

IPC分类号： B01L3/00 ,  G01N33/569

摘要： A test strip for detecting a serotype O foot-and-mouth disease, the test strip including: a bottom board; a detection layer being disposed on the bottom board and including a detection line and a control line; an absorbent layer being disposed at one end of the detection layer close to the control line; a gold colloidal conjugate pad being disposed at the other side of the detection layer close to the detection line; and a sample pad is disposed on a top of the gold colloidal conjugate pad. The gold colloidal conjugate pad is coated with colloidal gold particles that are conjugated with a Staphylococcus protein A (SPA) marker. The detection line is coated or impregnated with serotype O FMDV-like particles, and the control line is coated or impregnated with rabbit IgG.

公开(公告)号：US20190367885A1

公开(公告)日：2019-12-05

申请号：US15993637

申请日：2018-05-31

申请人： Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

发明人： Shiqi SUN ,  Huichen GUO ,  Yun ZHANG ,  Yanyan CHANG ,  Yanquan WEI ,  Jiaxi RU ,  Xiaoying ZHI ,  Ping DU ,  Xiangtao LIU ,  Hong YIN ,  Jianxun LUO

IPC分类号： C12N7/00 ,  G01N33/569

摘要： A method for preparing serotype O foot-and-mouth disease virus-like particles, the method including: construction of small ubiquitin-like modifier fusion expression vector, construction of recombinant expression vectors, construction of recombinant co-expression vector, expression and purification of proteins, and in-vitro assembly of serotype O foot-and-mouth disease virus-like particles. The disclosure also provides a test strip for detecting serotype O foot-and-mouth disease including a bottom board, and a detection layer disposed on the top of the bottom board. A detection line and a control line are disposed on the detection layer. An absorbent layer is disposed at one end of the detection layer close to the control line, and an immuno-gold pad is disposed at the other side of the detection layer close to the detection line. A sample pad is disposed on the top of the immuno-gold pad.

公开(公告)号：US20190008944A1

公开(公告)日：2019-01-10

申请号：US16027289

申请日：2018-07-04

申请人： Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences ,  Zhejiang University

发明人： Huichen GUO ,  Shiqi SUN ,  Ping DU ,  Zhidong TENG ,  Ruikang TANG ,  Ruibo ZHAO ,  Jiaxi RU ,  Yanquan WEI ,  Yun ZHANG ,  Yuan GAO ,  Junwu MA ,  Xiangtao LIU ,  Hong YIN

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5258 ,  C12N2770/32123 ,  C12N2770/32134

摘要： Foot-and-mouth disease virus like particles, the particles including a structural protein VP0, a structural protein VP1 including a mineralization peptide, a structural protein VP3, and a calcium phosphate coat. The structural protein VP1 including a mineralization peptide is encoded by a gene sequence represented by SEQ ID NO. 7, SEQ ID NO. 8 or SEQ ID NO. 9. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO. 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO. 3. The calcium phosphate coat covers the structural protein VP0, the structural protein VP1 including a mineralization peptide, and the structural protein VP3.