公开(公告)号：US4421772A

公开(公告)日：1983-12-20

申请号：US296612

申请日：1981-08-26

申请人： Lars Munck ,  Gregory C. Gibbons ,  Carol Feil

发明人： Lars Munck ,  Gregory C. Gibbons ,  Carol Feil

IPC分类号： G02B21/06 ,  B02C9/04 ,  B07C5/342 ,  G01J3/50 ,  G01N21/64 ,  G01N21/84 ,  G01N23/223 ,  G01J3/30 ,  G01N33/02

CPC分类号： G01N21/6456 ,  B02C9/04 ,  G01N23/223 ,  G01N2021/6417 ,  G01N2021/6423 ,  G01N2021/8466 ,  G01N2223/076 ,  Y10S250/91

摘要： In a method for identifying one or more botanical component parts from plants, a sample of the component parts is irradiated with electromagnetic radiation within the visible and/or ultra-violet and/or X-ray range for excitation of the component parts to fluorescence which is analyzed for determining characteristic fluorescence from the component parts. For determining the relative contents of the component parts in the sample, intensity measurements are carried out on the characteristic fluorescences or the number of emitters of characteristic fluorescence is counted. For identifying starchy endosperm parts, aleurone layer parts and hull layer parts originating from seeds, in particular cereal kernels, in ground seed samples, samples are excited at approximately 275 nm, approximately 350 nm and approximately 450 nm, respectively, for obtaining characteristic fluorescence at approximately 330 nm from the starchy endosperm parts, at approximately 420/470 nm from the aleurone layer parts, and at approximately 520 nm from the hull layer parts. Characteristic X-ray fluorescence is obtained from the potassium of the starchy endosperm, phosphorus and sulfur from the aleurone layer and silica from the hull layer. For manual evaluation of the component part contents, the characteristic fluorescences of the samples are compared with color scales. Fluorescence analysis provides more reliable qualitative and quantitative analyses of botanical component parts than do visible color analyses and ash and fiber analyses and is ideally suited for the control of component part separation processes such as mill processes.

摘要翻译： 在用于从植物鉴定一种或多种植物成分的方法中，将组分部分的样品用可见和/或紫外线和/或X射线范围内的电磁辐射照射，以将组分部分激发成荧光， 分析从组成部分确定特征荧光。 为了确定样品中组分部分的相对含量，对特征荧光进行强度测量，或对特征荧光的发射体数进行计数。 为了识别淀粉胚乳部分，来自种子，特别是谷粒的糊粉层部分和壳层部分在研磨种子样品中，样品分别在约275nm，约350nm和约450nm处被激发，以获得特征荧光 离淀粉胚乳部分大约330nm，距离糊粉层部分大约420/470nm，距离船体层部分约520nm。 特征X射线荧光从淀粉胚乳的钾，糊粉层中的磷和硫以及来自船体层的二氧化硅获得。 为了对组分部分内容进行手动评估，将样品的特征荧光与颜色比例进行比较。 荧光分析提供了比可见颜色分析和灰分和纤维分析更可靠的定性和定量分析植物组分部分，并且非常适合用于控制组分部分分离过程如研磨过程。

公开(公告)号：US4311358A

公开(公告)日：1982-01-19

申请号：US88320

申请日：1979-10-25

申请人： Gregory C. Gibbons ,  Lars Munck

发明人： Gregory C. Gibbons ,  Lars Munck

IPC分类号： B02C9/04 ,  G01N21/64 ,  G01N21/84 ,  G01N23/223 ,  G02B21/16

CPC分类号： G02B21/16 ,  B02C9/04 ,  G01N23/223 ,  G01N2021/8466 ,  G01N2223/076

摘要： An illumination device permitting microscopes, for use of the microscope as a fluorescence microscope, comprises a light-screening tube (2, 4) preferably of variable length, one end of the tube being connectible to the microscope objective and the other end being disposed to surround the sample which is intended for microscopic observation on the sample stage (1) of the microscope. An opening in the tube is arranged to direct high intensity UV and/or visible light towards the sample. In that the light is directed towards the sample bypassing the optics of the microscope, substantially unchanged light intensity will be realized from the light source to the sample, which considerably facilitates fluorescence studies with, in particular, microscope objectives of low magnifying power and low numerical aperture.

摘要翻译： 允许显微镜使用显微镜作为荧光显微镜的照明装置包括优选具有可变长度的遮光管（2,4），该管的一端可连接到显微镜物镜，另一端被设置为 围绕用于在显微镜的样品台（1）上进行显微镜观察的样品。 管中的开口被布置成朝向样品引导高强度的UV和/或可见光。 因为光被引向绕过显微镜的光学器件的样品，从光源到样品将实现基本上不变的光强度，这显着地促进了荧光研究，特别是具有低放大倍数和低数值的显微镜物镜 光圈。