公开(公告)号：US20060034767A1

公开(公告)日：2006-02-16

申请号：US10520431

申请日：2003-07-03

申请人： Lawrence Lum ,  Randall Lee ,  Nicola Kouttab

发明人： Lawrence Lum ,  Randall Lee ,  Nicola Kouttab

IPC分类号： A61K49/00 ,  A61K39/395

CPC分类号： G01N33/5005 ,  A61K49/0097 ,  A61K2035/124 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/2836 ,  C07K16/32 ,  C07K2317/31 ,  G01N33/505 ,  G01N33/5091 ,  G01N33/564 ,  G01N33/566 ,  G01N33/574

摘要： The present invention provides a method for tracking and targeting cells in viva. In particular, cells are isolated purified and armed with bispecific antibodies which are directly labeled with a detectable marker, or a second antibody specific for a region of the bispecific antibody is labeled with a detectable marker. The bispecific antibody is specific for a surface antigen of the cell and the second specificity of the specific antigen is for an antigen expressed on cells of tissues, organs or tumors. The armed and labeled cells can be tracked starting from the point of introduction into the animal until the cell reaches the target of interest by phenotyping cells from patient samples, obtained at different time intervals and locations, post-infusion. The isolated and purified cells are useful in any functional assay.

摘要翻译： 本发明提供了一种用于跟踪和靶向细胞的方法。 特别地，分离纯化细胞并用双特异性抗体装载，所述双特异性抗体用可检测标记物直接标记，或者用双重特异性抗体区域特异性的第二抗体用可检测标记物标记。 双特异性抗体对细胞的表面抗原是特异性的，特异性抗原的第二特异性是针对在组织，器官或肿瘤细胞上表达的抗原。 可以从引入到动物的角度开始追踪和标记的细胞，直到细胞通过表达来自患者样品的细胞达到感兴趣的目标，在不同的时间间隔和位置获得，在输注后。 分离和纯化的细胞可用于任何功能测定。