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公开(公告)号：US20210302316A1
公开(公告)日：2021-09-30
申请号：US17211924
申请日：2021-03-25
发明人： Kai WALTER , Lars FRIEDRICH
摘要： A method for estimating a stimulated emission depletion microscopy (STED) resolution includes generating a first frame representing a reference image from a field-of-view, the reference image having a predetermined reference resolution, and generating at least one second frame representing a STED image from the same field-of-view, the STED image having the STED resolution to be estimated. The at least one second frame is blurred by applying a convolution kernel with at least one fit parameter to the second frame. An optimal value of the at least one fit parameter of the convolution kernel is determined for which a difference between the first frame and the blurred at least one second frame is minimized. The STED resolution is estimated based on the optimal value of the at least one fit parameter and the predetermined reference resolution.
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公开(公告)号：US20190278072A1
公开(公告)日：2019-09-12
申请号：US16310472
申请日：2017-06-21
发明人： Florian FAHRBACH , Lars FRIEDRICH , Werner KNEBEL
摘要： An illumination apparatus for a microscope, for producing a de-excitation or switching light distribution, includes a light source configured to produce a primary illumination light beam and a beam splitter configured to divide the primary illumination light beam into two partial illumination light beams. An illumination objective is configured to focus the partial illumination light beams onto and/or into a sample such that the partial illumination light beams extend, spatially separated from one another, through an entry pupil of the illumination objective and are spatially superposed on and/or in the sample after passing through the illumination objective. A phase influencer is configured to cause a relative phase offset of the partial illumination light beams with respect to one another in such a way that the partial illumination light beams in the entry pupil of the illumination objective have a phase offset of π.
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公开(公告)号：US20230274392A1
公开(公告)日：2023-08-31
申请号：US18154890
申请日：2023-01-16
发明人： Kai WALTER , Lars FRIEDRICH , Florian ZIESCHE
IPC分类号： G06T3/40
CPC分类号： G06T3/4023
摘要： A digital image processing apparatus for computing a baseline estimate of a digital input image is provided. The digital image processing apparatus is configured to obtain a digital intermediate image by downsampling the digital input image by a predetermined downsampling factor, and compute the baseline estimate based on the digital intermediate image.
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4.发明申请公开(公告)号：US20190339500A1
公开(公告)日：2019-11-07
申请号：US16305902
申请日：2017-05-31
发明人： Bernd WIDZGOWSKI , Vishnu Vardhan KRISHNAMACHARI , Lars FRIEDRICH , Manuel KREMER , Arnold GISKE
IPC分类号： G02B21/00
摘要： An optical arrangement has an optical beam path for illuminating a sample space with a sequence of laser light pulses generated in a laser cycle, the optical arrangement. At least one laser light source is configured to generate the sequence of laser light pulses along the optical beam path. A wavelength-selective pulse picker is situated in the optical beam path and has, in a predefined illumination clock timing synchronizable with the laser light pulses, an open state in which the pulse picker is light-transparent to at least one laser light pulse towards the sample space. The open state has at least two different transmission states which differ with regard to their respective transmission spectrums, and wherein the two transmission states are switchable on and/or off independently of one another.
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公开(公告)号：US20220196555A1
公开(公告)日：2022-06-23
申请号：US17603596
申请日：2020-04-16
发明人： Jonas FOELLING , Lars FRIEDRICH
IPC分类号： G01N21/64
摘要： A fluorescence microscope includes excitation and de-excitation light sources designed to generate excitation and de-excitation light distributions, which excite and de-excite fluorophores present in a sample, respectively. An illumination unit is designed to combine the light distributions such that an intensity maximum of the excitation light distribution and an intensity minimum of the de-excitation light distribution are spatially superimposed on one another in an illumination target point. A detector is designed to detect the fluorescence photons as a function of their arrival times. The processor is designed to evaluate the detected fluorescence photons with respect to their arrival times and, based thereon, to control a delay which a light pulse or a light modulation of the de-excitation light distribution has at a position of the illumination target point in relation to a light pulse or a light modulation of the excitation light distribution.
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公开(公告)号：US20220196554A1
公开(公告)日：2022-06-23
申请号：US17603594
申请日：2020-04-17
发明人： Jonas FOELLING , Lars FRIEDRICH
IPC分类号： G01N21/64
摘要： A fluorescence scanning microscope includes excitation and de-excitation light sources, which are designed to generate an excitation and a de-excitation light distribution, respectively. An illumination unit combines the light distributions to form a light distribution scanning over multiple illumination target points of a sample in such a way that an intensity maximum of the excitation light distribution and an intensity minimum of the de-excitation light distribution are spatially superimposed on one another. A detector detects fluorescence photons emitted from the respective illumination target point as a function of their arrival times. A processor evaluates the fluorescence photons with respect to the arrival times, generates a first pixel and a second pixel based thereon, assembles the first and second pixels to form first and second sample images, respectively, and, by means of the two sample images, determines a spatial offset between the intensity maximum and the intensity minimum.
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