公开(公告)号：US20230059921A1

公开(公告)日：2023-02-23

申请号：US17453336

申请日：2021-11-02

申请人： Ligandal, Inc.

发明人： Andre Ronald Watson ,  Shahab Chizari ,  Ryan Spencer ,  Christian Foster ,  Shuailiang Lin ,  Sara Marie Peyrot ,  Pranali Deshpande ,  Matthew Dobbin ,  William Connors

IPC分类号： G01N33/566 ,  C40B30/04

摘要： Provided are methods and compositions for the heterologous expression of a payload (e.g., DNA, RNA, protein) of interest in a target cell (e.g., cancer cell). In some cases payload delivery results in expression (e.g., by a cancer cell in vivo) of a secreted immune signal such as a cytokine, a plasma membrane-tethered affinity marker (thus resulting in an induced immune response), or a cytotoxic protein such as an apoptosis inducer (e.g., by a cancer cell in vivo). Payloads are delivered with a delivery vehicle and in some cases the delivery vehicle is a nanoparticle. In some cases a subject nanoparticle includes a targeting ligand for targeted delivery to a specific cell type/tissue type (e.g., a cancerous tissue/cell). In some embodiments, payload delivery is “personalized” in the sense that the delivery vehicle and/or payload can be designed based on patient-specific information.

公开(公告)号：US20200208177A1

公开(公告)日：2020-07-02

申请号：US16387507

申请日：2019-04-17

申请人： Ligandal, Inc.

发明人： Andre Ronald Watson ,  Christian Foster ,  Shuailiang Lin ,  Sara Marie Peyrot

IPC分类号： C12N15/90 ,  C12N15/85 ,  C12N15/88

摘要： Provided are methods and compositions for genome editing using sticky ends. Subject methods include (a) generating a staggered cut at each of two locations in genomic DNA of a target cell, thus generating two genomic staggered ends; and (b) providing/introducing a linear double stranded donor DNA that has staggered ends (i.e., sticky ends) that match/correspond to the sticky ends of the genomic DNA such that the sticky ends of the donor DNA hybridize with the sticky ends of the genomic DNA and the donor DNA is inserted into the genome. In some cases, the staggered cuts are generated by introducing into a target cell one or more sequence specific nucleases (or one or more nucleic acids encoding the one or more sequence specific nucleases).