公开(公告)号：US20120302731A1

公开(公告)日：2012-11-29

申请号：US13522306

申请日：2010-10-14

申请人： Lin Li ,  Bing Li ,  Yingjuan Tian ,  Ling Chen ,  Liang Zhu ,  Guoqin Liu ,  Jianyu Su ,  Xiaoxi Li

发明人： Lin Li ,  Bing Li ,  Yingjuan Tian ,  Ling Chen ,  Liang Zhu ,  Guoqin Liu ,  Jianyu Su ,  Xiaoxi Li

IPC分类号： C07K2/00 ,  C07K1/34 ,  C12P21/06

CPC分类号： C12P21/06 ,  A23J3/34 ,  A61K38/00 ,  C07K2319/00 ,  C12F3/06

摘要： The present invention provides methods for the preparation of protein hydrolysate, peptide solution and peptide from BSG. The wet BSG or BSG powder is dispersed in extract solution to prepare the crude BSG protein or the crude BSG protein solution. Preparing the crude BSG protein solution using the crude BSG protein and adjusting the pH to 6.5˜8.5, or adjusting the pH of the crude BSG protein solution to 6.5˜8.5. Then the solution is hydrolyzed with protease at 45° C. to 65° C. for 1 h to 5 h in a water bath shaker to prepare BSG protein hydrolysate. The protein hydrolysate is heated to inactivate the protease and centrifuged to obtain the peptide solution. The peptide solution is separated by gel filtration and each peak is collected and pooled together to obtain the peptide. The protein hydrolysate, peptide solution and peptide in the present invention are all prepared from BSG which is a natural product and available at low cost throughout the year. There is no harmful material used in the production process. The results of in vitro experiment suggest that BSG peptide prepared by this method shows a significantly hypoglycemic effect.

摘要翻译： 本发明提供了从BSG制备蛋白质水解物，肽溶液和肽的方法。 将湿BSG或BSG粉末分散在提取液中以制备粗BSG蛋白或粗BSG蛋白溶液。 使用粗BSG蛋白制备粗BSG蛋白溶液并调节pH至6.5〜8.5，或将粗BSG蛋白溶液的pH调节至6.5〜8.5。 然后将溶液在45℃至65℃下用蛋白酶在水浴摇床中水解1小时至5小时以制备BSG蛋白水解产物。 将蛋白质水解产物加热灭活蛋白酶并离心得到肽溶液。 通过凝胶过滤分离肽溶液，并收集每个峰并将其汇合在一起以获得肽。 本发明中的蛋白质水解物，肽溶液和肽均由天然产物BSG制备，全年以低成本获得。 在生产过程中没有使用有害物质。 体外实验结果表明，通过该方法制备的BSG肽显示出明显的降血糖作用。